Evaluation of resveratrol's protective effect on hydrocortisone-induced growth inhibition in the peripubertal rat epiphyseal plate

• Published in: Tissue & cell 2024-12, Vol.91, p.102607, Article 102607
• Main Authors: Kemer, Serkan, Metin, Sefa, Celik, Ertugrul, Mamuk, Soner, Ergun, Hakan
• Format: Article
• Language: English
• Subjects: Animals; Apoptosis - drug effects; Corticosteroid; Epiphyseal plate; Female; Growth Plate - drug effects; Growth retardation; Hydrocortisone; Hydrocortisone - blood; Hydrocortisone - pharmacology; Rats; Rats, Wistar; Resveratrol; Resveratrol - pharmacology; Short stature; Tibia - diagnostic imaging; Tibia - drug effects; Tibia - growth & development
• ISSN: 0040-8166; 1532-3072
• DOI: 10.1016/j.tice.2024.102607

Chronic use of glucocorticoids during childhood can lead to a decrease in hormone release, including ACTH, GH, TSH, and LH, as well as reduced IGF-1 activity. This can result in osteoporotic changes and hinder growth in height. Resveratrol, an antioxidant with phytoestrogen properties, may improve bone health by increasing bone mineral density in postmenopausal women. It has been shown that resveratrol promotes osteoblastic bone formation and reduces osteoclastic resorption. We aimed to investigate the protective effects of resveratrol on the growth inhibition of the epiphyseal plate induced by hydrocortisone in peripubertal Wistar Albino rats. Rats were randomized into 6 groups and treated with hydrocortisone (10 mg/kg/day) and resveratrol (10–50–100 mg/kg/day) for 10 days after a standard AP tibia radiograph was taken. Blood and tibia bones were collected and evaluated for bone biomarkers (osteopontin, sclerostin), histopathological measures, and apoptosis markers. Subcutaneous administration of hydrocortisone for 10 days significantly reduced tibia linear growth, as evaluated by radiography (23.4 % vs. 15.1 %, p