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Analysis of PM 2.5 -induced cytotoxicity in human HaCaT cells based on a microfluidic system
Human exposure to PM causes several adverse health effects. Skin is the first barrier against harmful environmental substances and can directly contact with PM , but there is no study about PM -induced cytotoxicity in human skin cells on the molecular level partially due to the shortcomings of tradi...
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Published in: | Toxicology in vitro 2017-09, Vol.43, p.1-8 |
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Main Authors: | , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Human exposure to PM
causes several adverse health effects. Skin is the first barrier against harmful environmental substances and can directly contact with PM
, but there is no study about PM
-induced cytotoxicity in human skin cells on the molecular level partially due to the shortcomings of traditional research methods. In present study, we established a microfluidic system including a cell culture chip integrated with a high-throughput protein microarray chip to investigate the mechanism of PM
-mediated cytotoxicity in human HaCaT cells. We found that PM
was lodged inside the cytoplasm, mitochondria and nucleus of HaCaT cells by TEM. Flow cytometry analysis indicated that the cell apoptosis rate increased from 0.49% to 53.4%. The results of protein microarray showed that NF-κB and NALP3 signal transductions were activated in HaCaT cells after PM
stimulations, up-regulating the expression of IL-1β and IL-6, which resulted in inflammatory response in HaCaT cells. Our findings provide a molecular insight into PM
-induced skin injury. |
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ISSN: | 0887-2333 1879-3177 |
DOI: | 10.1016/j.tiv.2017.04.018 |