Analysis of genomic responses in a rat lung model treated with a humidifier sterilizer containing polyhexamethyleneguanidine phosphate

•We investigated the geno-toxicological effects of PHMG phosphate in the rat lung using a DNA microarray.•PHMG phosphate altered expression of genes involved in the urea cycle, inflammation, and oxidative stress.•21 genes were induced and 4 genes were repressed in response to PHMG phosphate in a tim...

Full description

Saved in:
Bibliographic Details
Published in:Toxicology letters 2017-02, Vol.268, p.36-43
Main Authors: Kim, Min-Seok, Jeong, Seok Won, Choi, Seong-Jin, Han, Jin-Young, Kim, Sung-Hwan, Yoon, Seokjoo, Oh, Jung-Hwa, Lee, Kyuhong
Format: Article
Language:English
Subjects:
Animals
Biomarkers
Disinfectants - toxicity
DNA microarray
Equipment Contamination - prevention & control
Female
Gene Expression Profiling - methods
Gene Expression Regulation
Guanidines - toxicity
Humidifier disinfectant
Humidifiers
Lung - drug effects
Lung - metabolism
Male
Oligonucleotide Array Sequence Analysis
PHMG phosphate
Pneumonia - chemically induced
Pneumonia - genetics
Pulmonary disease
Pulmonary Fibrosis - chemically induced
Pulmonary Fibrosis - genetics
Rats, Sprague-Dawley
Time Factors
Toxicogenetics - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:•We investigated the geno-toxicological effects of PHMG phosphate in the rat lung using a DNA microarray.•PHMG phosphate altered expression of genes involved in the urea cycle, inflammation, and oxidative stress.•21 genes were induced and 4 genes were repressed in response to PHMG phosphate in a timedependent manner. The antimicrobial biocide polyhexamethyleneguanidine (PHMG) phosphate is the main ingredient in the commercially available humidifier disinfectant. PHMG phosphate-based humidifier disinfectants can cause pulmonary fibrosis and induce inflammatory and fibrotic responses both in vivo and in vitro. However, toxicological mechanisms including genomic alterations induced by inhalation exposure to PHMG phosphate have not been elucidated. Therefore, this study evaluated the toxicological effects of the PHMG phosphate-containing humidifier disinfectant. We used DNA microarray to identify global gene expression changes in rats treated with PHMG phosphate-containing humidifier disinfectant for 4 weeks and 10 weeks. Functional significance of differentially expressed genes (DEGs) was estimated by gene ontology (GO) analysis. Four weeks post-exposure, 320 and 392 DEGs were identified in female and male rats, respectively (>2-fold, p
ISSN:0378-4274
1879-3169
DOI:10.1016/j.toxlet.2016.11.005