Advances in sample preparation and analytical techniques for lipidomics study of clinical samples

•Lipids are important biological molecules for the maintenance of a healthy body.•Clinical lipidomics helps to understand lipid-related human diseases.•Advances in sample preparation and mass spectrometry for clinical lipidomics.•Current key challenges and their potential solutions in clinical lipid...

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Bibliographic Details
Published in:TrAC, Trends in analytical chemistry (Regular ed.) Trends in analytical chemistry (Regular ed.), 2015-03, Vol.66, p.1-18
Main Authors: Teo, Chin Chye, Chong, William Pooi Kat, Tan, Eddy, Basri, Nurhidayah Binte, Low, Zhen Jie, Ho, Ying Swan
Format: Article
Language:English
Subjects:
Chromatography
Clinical lipidomics
Clinical sample analysis
Disease mechanism
Disease treatment
Lipid
Lipid extraction
Lipidomics
Mass spectrometry
Sample preparation
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Summary:•Lipids are important biological molecules for the maintenance of a healthy body.•Clinical lipidomics helps to understand lipid-related human diseases.•Advances in sample preparation and mass spectrometry for clinical lipidomics.•Current key challenges and their potential solutions in clinical lipidomics. Lipids play multiple key roles in biological systems, including acting as secondary energy reserves, being components of cellular membranes and participating in cell-signaling pathways. As a result, lipid defects have been implicated in the development of diseases. Consequently, the detailed analysis of lipids found in clinical samples, known as clinical lipidomics, is crucial for improving our understanding of disease mechanisms and progression, and the development of potential treatment strategies. This review documents the lipidomics workflow, beginning with a description of approaches to preparation of samples of lipids. We also describe progress in mass spectrometry technologies that led to the development of several widely-used techniques of lipid analysis. The review concludes with a discussion on the major challenges that exist in clinical lipidomics studies and looks at potential solutions that can address them.
ISSN:0165-9936
1879-3142
DOI:10.1016/j.trac.2014.10.010