Spatial distribution and stability of Gd0.6Eu0.4VO4 nanoparticles injected in mouse ear pinnae

• Published in: TrAC, Trends in analytical chemistry (Regular ed.) Trends in analytical chemistry (Regular ed.), 2025-01, Vol.182, p.118049, Article 118049
• Main Authors: Proiou, Eleni, Pinakidou, Fani, Paloura, Eleni C., Pétri, Nicolas, Gacoin, Thierry, Laplace-Builhé, Corinne, Schuck, Götz, Alexandrou, Antigoni, Katsikini, Maria
• Format: Article
• Language: English
• Subjects: EuVO4; GdVO4; Micro-X-Ray Absorption Fine Structure spectroscopy (μ-XAFS); Nanoparticles; Synchrotron; X-Ray Fluorescence (XRF) mapping
• ISSN: 0165-9936
• DOI: 10.1016/j.trac.2024.118049

X-Ray Fluorescence (XRF) mapping is employed for the study of the spatial distribution of GdVO4:Eu nanoparticles (NPs) after their injection into mouse ear pinnae. The injected NP colloids were detectable in a concentration range from 5 to 30 mM in vanadate ions (90–530 nM in NP concentrations). The distribution maps were recorded separately for Gd, Eu, and V and reveal that the three elements are collocalized, indicating the NP stability after the injection. The distribution pattern of the NPs is not homogeneous; they follow bifurcated paths of easy flow demonstrating the complexity of the tissue-colloid interactions. The V–K, Gd-L3 and Eu-L3-edge X-ray Absorption Fine Structure (XAFS) spectra of the NPs recorded prior to and after their injection confirm that the integrity of the nanoparticles is preserved after injection. This combined XRF/XAFS analysis paves the way for studies on the long-term fate of injected Gd-containing NPs in tissues. [Display omitted] •GdVO4:Eu NPs are studied before and after their injection in mouse ear tissues.•Detectable NP concentration range in mouse ear pinnae: 90–530 nM.•The NPs follow bifurcated paths of easy flow after their injection in the tissues.•Gd, Eu, and V atoms are collocated: The NPs retain their integrity.•The bonding environment and oxidation state of V, Gd and Eu do not change.