Albumin attenuates neutrophil activation induced by stimulators including antibodies against neutrophil-specific antigens

• Published in: Transfusion and apheresis science 2005-11, Vol.33 (3), p.289-298
• Main Authors: Hashimoto, Makoto, Saigo, Katsuyasu, Jyokei, Yukie, Kishimoto, Manami, Takenokuchi, Mariko, Araki, Nobuo, Imoto, Shion, Taniguchi, Kikuyo, Kumagai, Shunichi
• Format: Article
• Language: English
• Subjects: Albumin; Anti-HNA-1a antibody; Antibodies, Monoclonal - immunology; Antibodies, Monoclonal - pharmacology; Carcinogens - pharmacology; CD11b; CD11b Antigen - biosynthesis; Cells, Cultured; Granulocyte Colony-Stimulating Factor - pharmacology; Humans; L-selectin; L-Selectin - immunology; L-Selectin - metabolism; Membrane Glycoproteins - immunology; N-Formylmethionine Leucyl-Phenylalanine - pharmacology; Neutrophil; Neutrophil Activation - drug effects; Neutrophil Activation - immunology; Neutrophils - cytology; Neutrophils - immunology; Neutrophils - metabolism; Reactive oxygen species; Reactive Oxygen Species - metabolism; Serum Albumin - metabolism; Serum Albumin - pharmacology; Tetradecanoylphorbol Acetate - pharmacology
• ISSN: 1473-0502; 1878-1683
• DOI: 10.1016/j.transci.2005.08.001

As neutrophil activation is related to several adverse transfusion reactions, we studied about the activation induced by anti-neutrophil antibodies and the stabilizing effects of albumin pretreatment by means of flow cytometry. Anti-neutrophil monoclonal antibody (anti-HNA-1a, 1b, 2a) alone induced CD11b expression and shedding of L-selectin, and anti-HNA-1a reinforced reactive oxygen species (ROS) production. Albumin pretreatment significantly reduced CD11b expression and L-selectin shedding induced by fMLP and ROS production induced by PMA, G-CSF combined with PMA or LPS-fMLP, or anti-HNA-1a combined with PMA. These findings suggest that anti-HNA-1a is related to adverse reactions and albumin has a regulating effect on neutrophil activation.