An In Vitro Model of Cell Transplantation for Evaluation of Cell Engraftment Enhancers

Abstract The limited availability of organs for liver transplantation has focused interest on the use of cell transplants to restore hepatic function. Advances have been made in rodent models, but efficacy is limited in humans due to low engraftment efficiency. In rodents, pretransplantation treatme...

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Bibliographic Details
Published in:Transplantation proceedings 2009-07, Vol.41 (6), p.2487-2490
Main Authors: Alfaro, F.J, Grau, M, Ramírez, E, Cevey, M, Mellado, M, Castro, M.J, Meneu, J.C, Abradelo, M, Camañas, C, Moreno, E, Morales, P, Paz-Artal, E, Serrano, A
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Adhesion
Cell Culture Techniques - methods
Cell Transplantation - adverse effects
Cell Transplantation - methods
Embryonic Stem Cells - cytology
Embryonic Stem Cells - physiology
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Hepatocytes - transplantation
Humans
Liver - cytology
Medical sciences
Mice
Models, Animal
Surgery
Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases
Tissue, organ and graft immunology
Umbilical Veins - cytology
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Summary:Abstract The limited availability of organs for liver transplantation has focused interest on the use of cell transplants to restore hepatic function. Advances have been made in rodent models, but efficacy is limited in humans due to low engraftment efficiency. In rodents, pretransplantation treatment of the liver with engraftment enhancers (EE) shows that repopulation is feasible, although the toxicity of the substances impedes their application in humans. Evaluation of low-toxicity engraftment enhancers for human use requires testing in animal models, a time-consuming, expensive process that also raises ethical issues. To reduce animal use in the preliminary evaluation of a new EE, we designed an easily quantitated in vitro method that mimics an intraportal cell transplant. It is based on EE-mediated disruption of intercellular adhesion in confluent endothelial cell cultures.
ISSN:0041-1345
1873-2623
DOI:10.1016/j.transproceed.2009.05.010