Bioactive multilayer thin films of charged N, N-disubstituted hydrazine phosphorus dendrimers fabricated by layer-by-layer self-assembly

• Published in: Thin solid films 2008-01, Vol.516 (6), p.1256-1264
• Main Authors: Hernandez-Lopez, Jose-Luis, Khor, Hwei Ling, Caminade, Anne-Marie, Majoral, Jean-Pierre, Mittler, Silvia, Knoll, Wolfgang, Kim, Dong Ha
• Format: Article
• Language: English
• Subjects: Cell adhesion; Collective excitations (including excitons, polarons, plasmons and other charge-density excitations); Condensed matter: electronic structure, electrical, magnetic, and optical properties; Cross-disciplinary physics: materials science; rheology; Electronic structure and electrical properties of surfaces, interfaces, thin films and low-dimensional structures; Exact sciences and technology; Layer-by-layer self-assembly; Liquid phase epitaxy; deposition from liquid phases (melts, solutions, and surface layers on liquids); Materials science; Methods of deposition of films and coatings; film growth and epitaxy; Phosphorus dendrimer; Physics; Surface and interface electron states; Surface plasmon resonance
• ISSN: 0040-6090; 1879-2731
• DOI: 10.1016/j.tsf.2007.05.049

Charged N, N-disubstituted hydrazine phosphorus-containing dendrimers are deposited either as alternate all-dendrimers multilayers or alternating with linear polymers on 3-mercaptopropionic acid or 3-aminopropyldimethylethoxysilane coated surfaces via electrostatic layer-by-layer self-assembly. The behavior of the film formation is investigated by surface plasmon resonance spectroscopy and ellipsometry. Fetal cortical rat neurons were cultured on the dendrimer films in order to investigate the influence of the surface charge of the outermost layer on their adhesion and maturation. It was found that neurons attached preferentially and matured slightly faster on film surfaces terminated with positively charged dendrimers than on negatively charged surfaces.