Evaluation of acute kidney injury in dogs with complicated or uncomplicated Babesia rossi infection

• Published in: Ticks and tick-borne diseases 2020-05, Vol.11 (3), p.101406, Article 101406
• Main Authors: Defauw, P., Schoeman, J.P., Leisewitz, A.L., Goddard, A., Duchateau, L., Aresu, L., Meyer, E., Daminet, S.
• Format: Article
• Language: English
• Subjects: Acute Kidney Injury - parasitology; Acute Kidney Injury - physiopathology; Acute Kidney Injury - urine; Acute Kidney Injury - veterinary; AKI; Animals; Babesia - physiology; Babesiosis; Babesiosis - pathology; Babesiosis - physiopathology; Babesiosis - urine; Biomarkers - blood; Biomarkers - urine; Canine; Dog Diseases - pathology; Dog Diseases - physiopathology; Dog Diseases - urine; Dogs; Male; NGAL; Renal; SDMA
• ISSN: 1877-959X; 1877-9603
• DOI: 10.1016/j.ttbdis.2020.101406

Dogs with babesiosis can present with multiple complications, including acute kidney injury (AKI). The objective of this study was to characterize AKI in dogs with babesiosis caused by Babesia rossi at presentation and after treatment. Thirty-five client-owned dogs with B. rossi infection and 10 control dogs were included in this prospective observational study. Blood and urine were collected in Babesia-infected dogs at presentation (T0, n = 35), after 24 h (T24h, n = 11), and after 1 month (T1m, n = 9). The following urinary kidney injury biomarkers were assessed: urinary protein to creatinine ratio (UPC), urinary glomerular injury biomarkers (immunoglobulin G (uIgG) and C-reactive protein (uCRP)), and urinary tubular injury biomarkers (retinol-binding protein (uRBP) and neutrophil gelatinase-associated lipocalin (uNGAL)). Serum functional renal biomarkers were creatinine (sCr) and symmetric dimethylarginine (sSDMA). Post-mortem kidney biopsies were analyzed by light and transmission electron microscopy. At T0, all kidney injury biomarkers were significantly higher in Babesia-infected dogs compared to healthy controls (P  0.05). At T24h, all urinary tubular injury biomarkers and UPC decreased significantly (P  0.5). Significant changes in functional renal biomarkers were not seen after treatment (P > 0.05). Dogs with complicated babesiosis had significantly higher glomerular injury biomarkers, UPC, and sSDMA compared to uncomplicated cases (P  0.1). Dogs with babesiosis caused by B. rossi showed transient kidney injury, which was detected by all kidney injury biomarkers, but remained undetected by functional biomarkers. All infected dogs, irrespective of disease severity, suffered comparable kidney injury based on tubular injury biomarker concentrations, while loss of function was seen more often in dogs with complicated babesiosis based on sSDMA results.