Direct immobilization of cupredoxin azurin modified by site-directed mutagenesis on gold surface

For making efficient bioelectronic device, we have developed novel immobilization method of cupredoxin azurin modified on gold (Au) surface. A recombinant protein with cysteine residue by using site-directed mutagenesis was designed and then directly immobilized on Au surface without any chemical li...

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Bibliographic Details
Published in:Ultramicroscopy 2008-09, Vol.108 (10), p.1390-1395
Main Authors: Kim, Sang-Uk, Kim, Young Jun, Choi, Shin-Geon, Yea, Cheol-Heon, Singh, Ravindra P., Min, Junhong, Oh, Byung-Keun, Choi, Jeong-Woo
Format: Article
Language:English
Subjects:
Adsorption
Azurin - chemistry
Azurin - genetics
Cyclic voltammetry
Electrochemistry
Gold - chemistry
Microscopy, Scanning Tunneling - methods
Mutagenesis, Site-Directed - methods
Pseudomonas aeruginosa - enzymology
Pseudomonas aeruginosa - genetics
Scanning tunneling microscopy
Site-directed mutagenesis
Surface plasmon resonance
Surface Plasmon Resonance - methods
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Summary:For making efficient bioelectronic device, we have developed novel immobilization method of cupredoxin azurin modified on gold (Au) surface. A recombinant protein with cysteine residue by using site-directed mutagenesis was designed and then directly immobilized on Au surface without any chemical linker. The immobilization of the functionalized protein is confirmed by surface plasmon resonance (SPR) and its surface morphology is analyzed by scanning tunneling microscopy (STM). The immobilization efficiency has been increased about 75.6%, as compared to that of wild-type azurin. The electrochemical property of the fabricated thin film was investigated by the cyclic voltammetry (CV). As a result, cysteine-modified azurin can be used for making high-quality protein film, and applied to the fabrication of nano-scale bioelectronics.
ISSN:0304-3991
1879-2723
DOI:10.1016/j.ultramic.2008.04.048