Antibacterial and antifungal activity of silver nanospheres synthesized by tri-sodium citrate assisted chemical approach

Antimicrobial activity of silver nanoparticles, which were synthesized by tri-sodium citrate assisted chemical approach, was evaluated. Chemical approach used for the synthesis of silver nanoparticles it was based on diminishment of silver nitrate in nearness of trisodium citrate without using any o...

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Bibliographic Details
Published in:Vacuum 2017-12, Vol.146, p.259-265
Main Authors: Khatoon, Umme Thahira, Nageswara Rao, G.V.S., Mohan, Krishna M., Ramanaviciene, Almira, Ramanavicius, Arunas
Format: Article
Language:English
Subjects:
Antimicrobial activity
Fungus
Silver nanoparticles
Trisodium citrate
XRD spectra
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Summary:Antimicrobial activity of silver nanoparticles, which were synthesized by tri-sodium citrate assisted chemical approach, was evaluated. Chemical approach used for the synthesis of silver nanoparticles it was based on diminishment of silver nitrate in nearness of trisodium citrate without using any other capping and stabilizing agents. During the synthesis, four samples of silver nanoparticles were collected for comparative studies. The presence of silver nanoparticles was affirmed by analyzing the sample with X-ray diffraction, transmission electron microscopy, dynamic light scattering and ultra violet visible spectroscopy. Antimicrobial activity of some silver nanoparticle samples against two bacterial strains including Bacillus subtilis (which is Gram positive bacteria), Escherichia coli (which is Gram negative bacteria) and two fungal (yeast) strains namely Saccharomyce scerevisiae, and Candida albicans was determined. The silver nanoparticles showed the most significant inhibition activity against bacterial strains (Escherichia coli and Saccharomyces cerevisiae) in comparison to that activity towards fungal strains. •Synthesis of silver nanoparticles (AgNPs) of different size by advanced chemical approach was applied.•Phases of pure Ag were observed by UV-Vis spectroscopy, XRD and TEM-SAD.•Antimicrobial activity of AgNPs against several bacterial and several fungal strains was determined.•Highest sensitivity towards AgNPs was observed for Escherichia coli, Saccharomyces cerevesiae and Candida albicans.
ISSN:0042-207X
1879-2715
DOI:10.1016/j.vacuum.2017.10.003