Triterpenoid saponins affect the function of P-glycoprotein and reduce the survival of the free-living stages of Heligmosomoides bakeri

• Published in: Veterinary parasitology 2011-06, Vol.179 (1), p.144-151
• Main Authors: Doligalska, M., Jóźwicka, K., Kiersnowska, M., Mroczek, A., Pączkowski, C., Janiszowska, W.
• Format: Article
• Language: English
• Subjects: Animals; ATP-Binding Cassette, Sub-Family B, Member 1 - chemistry; ATP-Binding Cassette, Sub-Family B, Member 1 - pharmacology; Beta vulgaris; Beta vulgaris - chemistry; Calendula - chemistry; Calendula officinalis; eggs; Glucuronides - chemistry; Glucuronides - pharmacology; hatching; Heligmosomatoidea - drug effects; Heligmosomatoidea - physiology; Heligmosomoides; Heligmosomoides bakeri; intestines; Larva - drug effects; larvae; mice; molting; P-glycoprotein; Rhodamine 123; Saponins - chemistry; Saponins - pharmacology; thiabendazole; toxicity; transporters; Triterpenes - chemistry; Triterpenes - pharmacology; Triterpenoid saponins; Verapamil - pharmacology; xenobiotics
• ISSN: 0304-4017; 1873-2550
• DOI: 10.1016/j.vetpar.2011.01.053

We studied the effect of triterpenoid saponins on the development of free-living stages of Heligmosomoides bakeri, a parasitic nematode of the mouse intestine. We evaluated the effectiveness of oleane-type glucuronides (GlcUAOA) isolated from Calendula officinalis and Beta vulgaris. The rhodamine 123 retention assay was used to detect dysfunctions of the major membrane transporter for xenobiotics, P-glycoprotein (Pgp). Both C. officinals and B. vulgaris GlcUAOA affect the development of the free living stages and function of Pgp in H. bakeri. The GlcUAOA inhibits egg hatching and moulting of larvae and also changes their morphology. These saponin fractions reversed the toxic effect of thiabendazole on the nematode; the function of Pgp was also inhibited.