Detection of ivermectin resistance by a larval development test—Back to the past or a step forward?

The aim of this study was to evaluate the potential of the larval development test for the detection of ivermectin (IVM) resistance in Haemonchus contortus of sheep. Single infections with 5000 third-stage larvae of five resistant and two susceptible isolates of H. contortus were given to sheep. Fec...

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Bibliographic Details
Published in:Veterinary parasitology 2013-11, Vol.198 (1-2), p.154-158
Main Authors: Dolinská, M., Königová, A., Letková, V., Molnár, L., Várady, M.
Format: Article
Language:English
Subjects:
Animals
Anthelmintic resistance
Anthelmintics - pharmacology
Drug Resistance
eprinomectin
feces
Haemonchus - drug effects
Haemonchus contortus
In vitro detection
Ivermectin
Ivermectin - pharmacology
Larva - drug effects
larvae
larval development
lethal concentration 50
Lethal Dose 50
Sheep
Sheep Diseases - parasitology
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Summary:The aim of this study was to evaluate the potential of the larval development test for the detection of ivermectin (IVM) resistance in Haemonchus contortus of sheep. Single infections with 5000 third-stage larvae of five resistant and two susceptible isolates of H. contortus were given to sheep. Fecal samples were collected four times during patency, and the micro-agar version of the larval development test (MALDT) was performed. Three macrocyclic lactone drugs (IVM, eprinomectin and IVM aglycone) were tested. The results of the tests are presented as LC50 and LC99 values. The MALDT was well able to distinguish between susceptible and resistant isolates. Resistance factors (RF) for the LC99 values were generally higher than those obtained by comparing LC50 values. The highly resistant isolates were readily distinguishable from the susceptible isolates, particularly when using IVM aglycone and eprinomectin, with RFs above 20.
ISSN:0304-4017
1873-2550
DOI:10.1016/j.vetpar.2013.07.043