Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts

•Cytokine expression in adventitial layer is related to cyst fertility.•Co-infection with F. hepatica enhances cytokine expression in adventitial layer.•Cytokine expression was mostly localized to cells next to the laminated layer. Cystic echinococcosis is a widespread zoonosis caused by the larval...

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Published in:Veterinary parasitology 2021-02, Vol.290, p.109343, Article 109343
Main Authors: Jiménez, Mauricio, Hidalgo, Christian, Stoore, Caroll, Corrêa, Felipe, Pereira, Ismael, Hernández, Marcela, Sáenz, Leonardo, Benavides, Julio, Ferreras, M. Carmen, Royo, Marcos, Paredes, Rodolfo
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Language:English
Subjects:
Cytokines
Echinococcus granulosus
Fasciola hepatica
Immunohistochemistry
Ism
Local immune response
Polyparasit
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container_title Veterinary parasitology
container_volume 290
creator Jiménez, Mauricio
Hidalgo, Christian
Stoore, Caroll
Corrêa, Felipe
Pereira, Ismael
Hernández, Marcela
Sáenz, Leonardo
Benavides, Julio
Ferreras, M. Carmen
Royo, Marcos
Paredes, Rodolfo
description •Cytokine expression in adventitial layer is related to cyst fertility.•Co-infection with F. hepatica enhances cytokine expression in adventitial layer.•Cytokine expression was mostly localized to cells next to the laminated layer. Cystic echinococcosis is a widespread zoonosis caused by the larval stage of the tapeworm Echinococcus granulosus. In intermediary hosts, two types of echinococcal cysts can be found: fertile, which produce protoscoleces, the infective form of the parasite to dogs; and infertile, that do not present protoscoleces and therefore are not able to continue with the parasite life cycle. The adventitial layer, the local immune response against the cyst, plays an important role in cyst fertility. Grazing cattle can often feature Fasciola hepatica co-infection, a parasite known to modulate the host systemic immune response. In this work the cellular Th1/Th2 immune profiles were evaluated in the adventitial layer of fertile and non-fertile cysts with and without co-infection with Fasciola hepatica. Measuring with immunohistochemistry and qPCR in adventitial layer, we report that non-fertile cysts present higher levels of Th1 cytokines (IFN-γ (P 
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subjects Cytokines
Echinococcus granulosus
Fasciola hepatica
Immunohistochemistry
Ism
Local immune response
Polyparasit
title Fasciola hepatica co-infection enhances Th1 immune response in the adventitial layer of non-fertile Echinococcus granulosus cysts
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