Antiviral activity of phage display selected peptides against Porcine reproductive and respiratory syndrome virus in vitro

• Published in: Virology (New York, N.Y.) N.Y.), 2012-10, Vol.432 (1), p.73-80
• Main Authors: Liu, Ke, Feng, Xiuli, Ma, Zhiyong, Luo, Chao, Zhou, Bin, Cao, Ruibing, Huang, Li, Miao, Denian, Pang, Ran, He, Danni, Lian, Xue, Chen, Puyan
• Format: Article
• Language: English
• Subjects: Animals; Antiviral Agents - isolation & purification; Antiviral Agents - pharmacology; Antiviral peptide; Cell Line; China; Infectious Disease; Inhibitory Concentration 50; Microbial Sensitivity Tests; Peptide Library; Peptides - isolation & purification; Peptides - pharmacology; Phage display; Porcine reproductive and respiratory syndrome virus; Porcine respiratory and reproductive syndrome virus - drug effects; Swine; Viral Proteins - antagonists & inhibitors; Viral Proteins - genetics
• ISSN: 0042-6822; 1096-0341
• DOI: 10.1016/j.virol.2012.05.010

Abstract Porcine reproductive and respiratory syndrome is an important infectious disease of pigs and has a significant harmful effect on the livestock industry, especially in China. PRRSV ORF1b gene encodes primary proteins which play a vital role during PRRSV replication. In this paper, various 12-amino-acid peptides were displayed. These peptides could bind to the polymerase and helicase of PRRSV ORF1b protein, respectively, in which p9 exerted the highest antiviral activity with an IC50 of 56 μM, and the minimum toxicity to cells. It was proved that p9 inhibited PRRSV replication in infected MARC-145 cells in a dose-dependent manner, and the amino acid sequence of HRILMRIR was important for antiviral activity of p9. Also, p9 could bind to the cell membrane and penetrated into cells. These result suggested that p9 might be a potential therapeutic drug for PRRSV infection.