Genomic characteristics and pathogenicity of a new bat adenoviruses strains that was isolated in at sites along the southeastern coasts of the P. R. of China from 2015 to 2019

•Finding a new adenovirus in the genus Mastadenovirus.•Sampling and collecting 552 bat samples in the southeastern coast of China.•Highly variable E3 sequence proves that bat adenovirus has high genetic diversity. Bats are important reservoirs for many kinds of emerging zoonotic viruses. In order to...

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Published in:Virus research 2022-01, Vol.308, p.198653, Article 198653
Main Authors: Ai, Lele, Zhu, Changqiang, Zhang, Wen, He, Ting, Ke, Yuehua, Wu, Jiahong, Yin, Wen, Zou, Xiaobo, Ding, Chenxi, Luo, Yizhe, Wang, Changjun, Qian, Hui, Tan, Weilong
Format: Article
Language:English
Subjects:
Adenoviridae - genetics
Adenoviridae Infections - epidemiology
Adenovirus
Animals
Bat
China
Chiroptera
Genome, Viral
Mastadenovirus
NGS
Pathogenicity
Phylogeny
Virulence
Zoonotic viruses
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Summary:•Finding a new adenovirus in the genus Mastadenovirus.•Sampling and collecting 552 bat samples in the southeastern coast of China.•Highly variable E3 sequence proves that bat adenovirus has high genetic diversity. Bats are important reservoirs for many kinds of emerging zoonotic viruses. In order to explore potential pathogens carried by bats and trace the source of adenovirus outbreaks on the southeastern coast of China, we took pharyngeal and anal swabs from a total of 552 bats (Rhinolophus pusillus) collected from various areas of Chinese southeastern coast. Adenoviruses were identified in 36 out of the 552 samples (6.5%) . Complete genome sequences of two adenovirus isolations from Vero E6 cells were obtained, which were further validated as identical strains via next-generation sequencing and were named Bat-Advcxc6. The cell culture inoculated with the two samples exhibited remarkable cytopathic changes. The full genome has 37,315 bp and owns 29 open reading frames. Phylogenetic analyses confirmed that Bat-Advcxc6 represented a novel bat adenovirus species in the genus Mastadenovirus. Transmission electron microgram showed clear virus particles. Bat-Advcxc6 shared similar characteristics of G + C contents with Bat mastadenovirus WIV11 (Bat mastadenovirus C) found in China in 2016, but differed from this serotype due to a
ISSN:0168-1702
1872-7492
DOI:10.1016/j.virusres.2021.198653