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Metarhodopsin-II stabilization by crosslinked Gt α C-terminal peptides and implications for the mechanism of GPCR-G protein coupling

Metarhodopsin-II is the light-excited form of rhodopsin that triggers G protein function. Metarhodopsin-II is stabilized when the N-terminus of the carboxyl (340–350) tail peptide of the α-subunit of transducin (Gtα) is crosslinked to rhodopsin cysteine 140 or the 340–350 peptide C-terminus of Gtα i...

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Bibliographic Details
Published in:Vision research (Oxford) 2006-12, Vol.46 (27), p.4547-4555
Main Authors: Angel, Thomas E., Kraft, Paul C., Dratz, Edward A.
Format: Article
Language:English
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Summary:Metarhodopsin-II is the light-excited form of rhodopsin that triggers G protein function. Metarhodopsin-II is stabilized when the N-terminus of the carboxyl (340–350) tail peptide of the α-subunit of transducin (Gtα) is crosslinked to rhodopsin cysteine 140 or the 340–350 peptide C-terminus of Gtα is crosslinked to rhodopsin cysteine 316. When the N-terminus of the peptide is coupled to C316 the MI ↔ MII equilibrium is not affected. The evidence suggests that the N-terminus of the 340–350 region of Gtα is located near C140 when transducin stabilizes metarhodopsin-II and alternative explanations are suggested for the effectiveness of the 340–350 Gtα tail peptide when bound to C316.
ISSN:0042-6989
1878-5646
DOI:10.1016/j.visres.2006.08.015