Performance evaluation of biocatalytic and biostimulation approaches for the remediation of trace organic contaminants in municipal biosolids

• Published in: Waste management (Elmsford) 2021-02, Vol.120, p.373-381
• Main Authors: Vaithyanathan, Vasanth Kumar, Savary, Olivier, Cabana, Hubert
• Format: Article
• Language: English
• Subjects: Biodegradation, Environmental; Bioremediation; Biosolids; Biostimulation; Enzyme cocktail; Hydrolysis; Laccase; Municipal biosolids; Trace organic contaminants
• ISSN: 0956-053X; 1879-2456
• DOI: 10.1016/j.wasman.2020.11.046

[Display omitted] •10 organic contaminants (concentration of 167 ± 14 µg/kg) were present in biosolids.•Biosolids was valorised by indigenous microbes to remove the organic contaminants.•Comparing the efficacy of various treatments on contaminants removal were studied.•Indigenous microbes present in the biosolids removed 20% of organic contaminants.•Addition of glucose and enzyme cocktail prior to digestion showed the best removal. Trace organic contaminants (TrOCs) in biosolids is creating potential threats for reuse of biosolids. Out of the tested 64 trace organic contaminants, seven pharmaceutically active compounds (PhACs), and three pesticides were detected in biosolids from a municipal wastewater treatment plant. This study encompasses the removal of TrOCs and improvement in the aerobic digestion of biosolids by various pretreatments including utilization of indigenous microbes present in biosolids (T1), the effect of an enzymatic pretreatment (T2), biostimulation by the addition of an external carbon source (T3) and the synergic effect of biostimulation and enzymatic pretreatment (T4). After 28 days of aerobic digestion, total PhACs removal was 44% with T1, which improved to 51%, 54% and 62% in T2, T3 and T4, respectively. Also, total pesticides removal was 10% in T1, which enhanced to 44%, 14% and 54% in T2, T3 and T4, respectively. The extracellular enzyme activities were also monitored in all the treatments and the maximum activities (114 ± 11 U/L lipase, 382 ± 29 U/L phosphatase, 155 ± 8 U/L protease, 304 ± 26 U/L amylase, 108 ± 7 U/L laccase, and 63 ± 2 U/L lignin peroxidase) were observed in T4 after 28 days of digestion. Thus, this study aids in providing changing aspects of enzyme profiles during these processes and the enhanced bioremediation of biosolids through the hydrolytic and oxidoreductase enzymes produced by the indigenous microorganisms.