Platelet-derived exosomes hybrid liposomes facilitate uninterrupted singlet oxygen generation to enhance breast cancer immunotherapy

• Published in: Cell reports physical science 2023-07, Vol.4 (7), p.101505, Article 101505
• Main Authors: Ning, Shipeng, Zhang, Xing, Suo, Meng, Lyu, Meng, Pan, You, Jiang, Yi, Yang, Huawei, Yip Lam, Jacky Wing, Zhang, Tianfu, Pan, Linghui, Tang, Ben Zhong
• Format: Article
• Language: English
• Subjects: aggregation-induced emission photosensitizer; enzyme dynamic therapy; immunotherapy; platelet-derived exosome hybrid liposome; uninterrupted singlet oxygen generation
• ISSN: 2666-3864; 2666-3864
• DOI: 10.1016/j.xcrp.2023.101505

The development of strategies to maintain the long-term presence of reactive oxygen species in tumor sites remains an urgent issue to be addressed. Here, we design platelet exosome hybrid liposome nanovesicles (named platelet-derived exosome hybrid liposomes co-loaded with DPDPy and chloroperoxidase [DCHL]) co-loading aggregation-induced emission (AIE) photosensitizers and chloroperoxidases to facilitate uninterrupted singlet oxygen (1O2) generation for enhancing breast cancer immunotherapy. When DCHL enters tumor cells, it lyses under external laser irradiation, thus releasing AIE photosensitizers (DPDPy) and chloroperoxidase. Subsequently, chloroperoxidase utilizes hydrogen peroxide (H2O2) and chloride to synthesize HClO, and, as a second step, HClO reacts with H2O2 to form 1O2. Therefore, uninterrupted 1O2 generation is achieved. The experimental results show that DCHL plus light can lead to systemic immune response, increase the proportion of central memory T cells and tumor-infiltrating T cells, and thereby delay tumor recurrence and rechallenge. Overall, the DCHL system provides a good idea for the design of tumor treatment system. [Display omitted] •Hybrid liposome nanovesicles enable uninterrupted 1O2 generation•The DCHL treatment induces anti-tumor immune response•This strategy inhibits tumor recurrence and rechallenge To facilitate uninterrupted 1O2 generation, Ning et al. design platelet exosome hybrid liposome nanovesicles co-loading AIE photosensitizers and chloroperoxidases. The nanovesicles can lead to systemic immune response and increase the proportion of central memory T cells and tumor-infiltrating T cells, thereby inhibiting tumor recurrence and rechallenge.