ALDH1A1+ ovarian cancer stem cells co-expressing surface markers CD24, EPHA1 and CD9 form tumours in vivo

One of the reasons for recurrence following treatment of high grade serous ovarian carcinoma (HGSOC) is the persistence of residual cancer stem cells (CSCs). There has been variability between laboratories in the identification of CSC markers for HGSOC. We have identified new surface markers (CD24,...

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Bibliographic Details
Published in:Experimental cell research 2020-07, Vol.392 (1), p.112009, Article 112009
Main Authors: Nagare, Rohit P., Sneha, Smarakan, Krishnapriya, Syama, Ramachandran, Balaji, Murhekar, Kanchan, Vasudevan, Sekar, Shabna, Aboo, Ganesan, Trivadi S.
Format: Article
Language:English
Subjects:
ALDEFLUOR assay
Aldehyde Dehydrogenase 1 Family - genetics
Aldehyde Dehydrogenase 1 Family - metabolism
Animals
Antigens, Surface - genetics
Antigens, Surface - metabolism
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
CD24
CD24 Antigen - genetics
CD24 Antigen - metabolism
CD9
Cell Line, Tumor
Cell Transformation, Neoplastic - genetics
Cell Transformation, Neoplastic - metabolism
Composite approach
Cystadenocarcinoma, Serous - genetics
Cystadenocarcinoma, Serous - metabolism
Cystadenocarcinoma, Serous - pathology
EPHA1
Female
Heterografts
Humans
Mice
Mice, Nude
Mice, Transgenic
Neoplasm Invasiveness
Neoplastic Stem Cells - metabolism
Neoplastic Stem Cells - pathology
Neoplastic Stem Cells - physiology
Ovarian cancer stem cells
Ovarian Neoplasms - genetics
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
Receptor, EphA1 - genetics
Receptor, EphA1 - metabolism
Retinal Dehydrogenase - genetics
Retinal Dehydrogenase - metabolism
Tetraspanin 29 - genetics
Tetraspanin 29 - metabolism
Xenograft assay
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Summary:One of the reasons for recurrence following treatment of high grade serous ovarian carcinoma (HGSOC) is the persistence of residual cancer stem cells (CSCs). There has been variability between laboratories in the identification of CSC markers for HGSOC. We have identified new surface markers (CD24, CD9 and EPHA1) in addition to those previously known (CD44, CD117 and CD133) using a bioinformatics approach. The expression of these surface markers was evaluated in ovarian cancer cell lines, primary malignant cells (PMCs), normal ovary and HGSOC. There was no preferential expression of any of the markers or a combination. All the markers were expressed at variable levels in ovarian cancer cell lines and PMCs. Only CD117 and CD9 were expressed in the normal ovarian surface epithelium and fallopian tube. Both ALDEFLUOR (ALDH1A1) and side population assays identified a small proportion of cells (
ISSN:0014-4827
1090-2422
DOI:10.1016/j.yexcr.2020.112009