Genome-wide identification, characterization and expression analysis of the TLP gene family in melon (Cucumis melo L.)

• Published in: Genomics (San Diego, Calif.) Calif.), 2020-05, Vol.112 (3), p.2499-2509
• Main Authors: Liu, Yarong, Cui, Jun, Zhou, Xiaoxu, Luan, Yushi, Luan, Feishi
• Format: Article
• Language: English
• Subjects: Ascomycota; Chromosomes, Plant; Cucumis melo - genetics; Cucumis melo - growth & development; Cucumis melo - metabolism; Gene Duplication; Genome, Plant; Genome-wide; Melon; Multigene Family; Phylogeny; Plant Diseases - genetics; Plant Diseases - microbiology; Plant Proteins - chemistry; Plant Proteins - classification; Plant Proteins - genetics; Plant Proteins - metabolism; Powdery mildew; PR5; Resistance; Sequence Alignment; Sequence Analysis, Protein; Thaumatin-like protein
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1016/j.ygeno.2020.02.001

Thaumatin-like proteins (TLPs), which belong to pathogenesis-related (PR) protein family 5 (PR5), are involved in plant host defense and various developmental processes. The functions of the TLP family have been extensively discussed in multiple organisms, whereas the detailed information of this family in melon has not been reported yet. In this study, we identified 28 TLP genes in the melon genome and a N-terminal signal peptide was found highly conserved within each member of this family. Phylogeny analysis indicated that TLPs from melon and other plant species were clustered into ten groups. Twelve segmental and seven tandem duplication gene pairs that underwent purifying selection were identified. TLP genes expressed differentially in different tissues/organs, and were significantly induced after Podosphaera xanthii infection. TLPs in breeding line MR-1 tend to express early after pathogen infection compared with cultivar Top Mark. Our study provides a comprehensive understanding of the melon TLP family and demonstrates their potential roles in disease resistance, therefore provides more reference for further research. •28 TLPs were identified in melon.•TLPs from melon and other plant species were clustered into ten groups.•The expression of most TLP genes were modulated by Px infection, and exhibited certain cultivar-specific pattern.