Integrated human papillomavirus types 52 and 58 are infrequently found in cervical cancer, and high viral loads predict risk of cervical cancer

• Published in: Gynecologic oncology 2006-07, Vol.102 (1), p.54-60
• Main Authors: Ho, Chih-Ming, Chien, Tsai-Yen, Huang, Shih-Hung, Lee, Bor-Heng, Chang, Shwu-Fen
• Format: Article
• Language: English
• Subjects: Cervical intraepithelial neoplasia; Cervical Intraepithelial Neoplasia - genetics; Cervical Intraepithelial Neoplasia - pathology; Cervical Intraepithelial Neoplasia - virology; DNA, Viral - genetics; DNA-Binding Proteins - genetics; Female; HPV; Humans; Integration; Neoplasm Staging; Oncogene Proteins, Viral - genetics; Papillomaviridae - genetics; Papillomaviridae - physiology; Papillomavirus Infections - complications; Papillomavirus Infections - virology; Polymerase chain reaction; Prospective Studies; Repressor Proteins - genetics; Reverse Transcriptase Polymerase Chain Reaction; Risk Factors; Uterine Cervical Neoplasms - genetics; Uterine Cervical Neoplasms - pathology; Uterine Cervical Neoplasms - virology; Viral Load; Virus Integration
• ISSN: 0090-8258; 1095-6859
• DOI: 10.1016/j.ygyno.2005.11.035

The aim of this prospective study was to analyze whether integration or high viral loads of human papillomavirus (HPV) is essential for malignant transformation of HPV types 52 and 58 as well as types 16 and 18. Cervical swabs from 178 consecutive patients, including 81 with invasive cervical cancers and 97 with cervical intraepithelial neoplasias (CIN) II–III, were collected and examined to determine the physical status and viral load of HPV types 16, 18, 52 and 58 DNA using genechip and real-time PCR (polymerase chain reaction) analysis. In cervical cancer patients, the integrated form of HPV 52 and 58 DNA was found in 25.0% and 12.5% of swabs, respectively; while HPV16 and 18 DNA was found in 82.6% and 100% of swabs, respectively ( P