The fractionated Toona sinensis leaf extract induces apoptosis of human ovarian cancer cells and inhibits tumor growth in a murine xenograft model

Aqueous extract from the leaves of Toona sinensis Roem. has been shown to have an anti-proliferative effect on human lung cancer cells. In this study, we analyzed the anti-cancer activity/effect of different extraction fractions of the extract from T. sinensis leaves on ovarian cancer cells. Cell vi...

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Published in:Gynecologic oncology 2006-08, Vol.102 (2), p.309-314
Main Authors: Chang, Hsueh-Ling, Hsu, Hseng-Kuang, Su, Jinu-Huang, Wang, Pei-Hui, Chung, Yueh-Fang, Chia, Yi-Chen, Tsai, Li-Yu, Wu, Yang-Chang, Yuan, Shyng-Shiou F.
Format: Article
Language:English
Subjects:
Animals
Apoptosis
Apoptosis - drug effects
Cedrela - chemistry
Cell cycle
Cell Cycle - drug effects
Cell Cycle Proteins - metabolism
Cell Line, Tumor
Female
HeLa Cells
Humans
Mice
Mice, Nude
Ovarian cancer
Ovarian Neoplasms - drug therapy
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
Plant Extracts - isolation & purification
Plant Extracts - pharmacology
Plant Leaves - chemistry
Toona sinensis
Trees - chemistry
Xenograft Model Antitumor Assays
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Summary:Aqueous extract from the leaves of Toona sinensis Roem. has been shown to have an anti-proliferative effect on human lung cancer cells. In this study, we analyzed the anti-cancer activity/effect of different extraction fractions of the extract from T. sinensis leaves on ovarian cancer cells. Cell viability was determined by XTT cell proliferation assay and cell survival assay. Apoptotic effect was detected by morphological analysis and immunoblotting. Cell cycle effect was evaluated by flow cytometry analysis and immunoblotting. In vivo therapeutic effect was evaluated by the subcutaneous inoculation of SKOV3 cells in nude mice (Foxnlnu/Foxnlnu) model. TSL2 of T. sinensis was more cytotoxic than other fractions and exhibited selectivity for ovarian cancer cell lines. TSL2 arrested SKOV3 ovarian cancer cells at the G2/M phase and induced cancer cells go through apoptotic pathway. Ex vivo xenograft study of nude mice showed that intraperitoneal injection of TSL2 was able to suppress the proliferation of ovarian cancer cells without significant nephrotoxicity, liver toxicity, or bone marrow suppression.
ISSN:0090-8258
1095-6859
DOI:10.1016/j.ygyno.2005.12.023