Serine recombinases as tools for genome engineering

The serine recombinases differ mechanistically from the tyrosine recombinases and include proteins such as ϕC31 integrase which, unlike Cre and Flp, promote unidirectional reactions. The serine recombinase family is large and includes many other proteins besides ϕC31 integrase with the potential to...

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Bibliographic Details
Published in:Methods (San Diego, Calif.) Calif.), 2011-04, Vol.53 (4), p.372-379
Main Authors: Brown, William R.A., Lee, Nicholas C.O., Xu, Zhengyao, Smith, Margaret C.M.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Culture Techniques
Gene Deletion
Genetic Engineering - methods
Genome
Humans
Integrases - genetics
Integrases - metabolism
Molecular Sequence Data
Protein Sorting Signals
Protein Structure, Quaternary
Protein Structure, Tertiary
Recombinases - chemistry
Recombinases - genetics
Recombinases - metabolism
Recombination, Genetic
Serine recombinases
Site-specific recombination
Transfection - methods
Unidirectional
ϕC31 integrase
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Summary:The serine recombinases differ mechanistically from the tyrosine recombinases and include proteins such as ϕC31 integrase which, unlike Cre and Flp, promote unidirectional reactions. The serine recombinase family is large and includes many other proteins besides ϕC31 integrase with the potential to be widely used in genome engineering. Here we review the details of the mechanism of the reactions promoted by the serine recombinases and discuss how these not only limit the utility of this class of recombinase but also creates opportunities for the engineering of new enzymes. We discuss the unanswered questions posed by genome engineering experiments in a variety of systems in which the serine recombinases have been used and finally describe more recently discovered serine recombinases that have the potential to be used in genome engineering.
ISSN:1046-2023
1095-9130
DOI:10.1016/j.ymeth.2010.12.031