Cu,Zn-Superoxide Dismutase Is an Intracellular Catalyst for the H₂O₂-dependent Oxidation of Dichlorodihydrofluorescein

Dichlorodihydrofluorescein (DCFH₂) is a widely used probe for intracellular H₂O₂. However, H₂O₂ can oxidize DCFH₂ only in the presence of a catalyst, whose identity in cells has not been clearly defined. We compared the peroxidase activity of Cu,Zn-superoxide dismutase (CuZnSOD), cytochrome c, horse...

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Published in:Molecules and cells 2006-02, Vol.21 (1), p.161-165
Main Authors: Kim, Y.M. (Kangwon National University, Chuncheon, Republic of Korea), Lim, J.M. (Kangwon National University, Chuncheon, Republic of Korea), Kim, B.C. (Kangwon National University, Chuncheon, Republic of Korea), Han, S.H. (Kangwon National University, Chuncheon, Republic of Korea), E-mail: hansh@kangwon.ac.kr
Format: Article
Language:English
Subjects:
Cu,Zn-Superoxide Dismutase
Dichlorodihydrofluorescein
HYDROGEN PEROXIDE
PEROXIDASAS
PEROXIDASES
PEROXIDO DE HIDROGENO
PEROXYDASE
PEROXYDE D'HYDROGENE
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Summary:Dichlorodihydrofluorescein (DCFH₂) is a widely used probe for intracellular H₂O₂. However, H₂O₂ can oxidize DCFH₂ only in the presence of a catalyst, whose identity in cells has not been clearly defined. We compared the peroxidase activity of Cu,Zn-superoxide dismutase (CuZnSOD), cytochrome c, horseradish peroxidase (HRP), Cu²+, and Fe³+ under various conditions to identify an intracellular catalyst. Enormous increase by bicarbonate in the rate of DCFH₂ oxidation distinguished CuZnSOD from cytochrome c and HRP.
ISSN:1016-8478
DOI:10.1016/s1016-8478(23)12915-3