Early morphological and chemical changes induced by cisplatin in the guinea pig organ of Corti

Guinea pigs were treated with a single dose of Cisplatin (5 mg IP). After 2–4 days the cochleas were prepared for morphological analysis by scanning electron microscopy and chemical analysis by X-ray dispersive microanalysis. Following Cisplatin, the bundles of stereocilia on the hair cells were fou...

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Bibliographic Details
Published in:Journal of laryngology and otology 1986-12, Vol.100 (12), p.1375-1383
Main Authors: Comis, Spiro D., Rhys-Evans, Peter H., Osborne, Michel P., Pickles, James O., Jeffries, David J. R., Pearse, Henry A. C.
Format: Article
Language:English
Subjects:
Animals
Calcium - metabolism
Cilia - drug effects
Cisplatin - toxicity
Electron Probe Microanalysis
Guinea Pigs
Hair Cells, Auditory - drug effects
Hair Cells, Auditory - metabolism
Hair Cells, Auditory - ultrastructure
Hair Cells, Auditory, Inner - drug effects
Hair Cells, Auditory, Inner - ultrastructure
Microscopy, Electron, Scanning
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Summary:Guinea pigs were treated with a single dose of Cisplatin (5 mg IP). After 2–4 days the cochleas were prepared for morphological analysis by scanning electron microscopy and chemical analysis by X-ray dispersive microanalysis. Following Cisplatin, the bundles of stereocilia on the hair cells were found to be rough, disarrayed, fused, and finally absorbed. Significant increases were found in the levels of calcium, sulphur, and phosporus in the abnormal hair cells. It is suggested that the high calcium levels might be due to the inhibition of enzymes which normally keep cytoplasmic calcium low, and that some of the changes in the stereocilia might be secondary to this.
ISSN:0022-2151
1748-5460
DOI:10.1017/S0022215100101161