Determination of Herbicide Inhibition of Photosynthetic Electron Transport by Fluorescence

The kinetics of chlorophyll (Chl) fluorescence was used as a tool for detecting herbicide inhibition in studies using intact soybean [Glycine max (L.) Merr.] leaves. The terminal level of fluorescence (FT), obtained 150 s after the onset of illumination of the abaxial leaf surface, was found to be i...

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Bibliographic Details
Published in:Weed science 1983-05, Vol.31 (3), p.361-367
Main Authors: Richard, Edward P., Goss, John R., Arntzen, Charles J., Slife, Fred W.
Format: Article
Language:English
Subjects:
Chlorophylls
Electrons
Fluorescence
Herbicides
Kinetics
Leaves
Optical filters
Photosynthesis
Plants
Soybeans
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Summary:The kinetics of chlorophyll (Chl) fluorescence was used as a tool for detecting herbicide inhibition in studies using intact soybean [Glycine max (L.) Merr.] leaves. The terminal level of fluorescence (FT), obtained 150 s after the onset of illumination of the abaxial leaf surface, was found to be independent of the dark preadaptation interval and to vary little between leaflets and leaves within and among untreated plants. Increases in FT were detected in plants following the foliar application of herbicides which inhibit photosynthetic electron transport. Fluorescence measurements indicated significant electron transport inhibition in leaves following treatment with 40-mM solutions of either atrazine [2-chloro-4-(ethylamino)-6-(isopropyiamino)-s-triazine] or diuron [3-(3,4-dichlorophenyl)-1,1-dimethylurea] after 0.5 and 1 h, respectively. Results of this study indicate that Chl fluorescence can be used to measure injury qualitatively by photosynthetic electron transport-inhibiting herbicides in intact plants long before visual symptoms of injury occur. Possible uses of this sensitive, rapid, and non-destructive technique for studying herbicide penetration as affected by adjuvants and environmental factors are discussed.
ISSN:0043-1745
1550-2759
DOI:10.1017/S0043174500069174