RAPD analysis of cultivated and wild yellow nutsedge (Cyperus esculentus L.)

Cultivated and weedy clones of yellow nutsedge were analyzed using random amplified polymorphic DNA (RAPD) markers to assess the polymorphism within the species and determine if this approach was suitable for identification of cultivar and wild populations. The RAPD markers unambiguously identified...

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Bibliographic Details
Published in:Weed science 1998-05, Vol.46 (3), p.318-321
Main Authors: Abad, Paloma, Pascual, Bernardo, Maroto, José V., López-Galarza, Salvador, Vicente, María J., Alagarda, José
Format: Article
Language:English
Subjects:
CLONE
CLONES
CROP YIELD
Crops
CULTIVAR IDENTIFICATION
CULTIVARS
CYPERUS ESCULENTUS
DNA
ENSAYO DE SEMILLAS
ESSAI DE SEMENCES
GENETIC DIVERSITY
GENETIC MARKERS
GENETIC POLYMORPHISM
GENETIC VARIATION
MARCADORES GENETICOS
MARQUEUR GENETIQUE
Operons
Plants
POLIMORFISMO GENETICO
POLYMORPHISME GENETIQUE
Population genetics
POPULATIONS
QUANTITATIVE TRAITS
RANDOM AMPLIFIED POLYMORPHIC DNA
RAPD
RENDEMENT DES CULTURES
RENDIMIENTO DE CULTIVOS
SEED TESTING
TUBERCULE
TUBERCULO
TUBERS
VARIACION GENETICA
VARIATION GENETIQUE
VARIEDADES
VARIETE
VARIETIES
Weed Biology and Ecology
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Summary:Cultivated and weedy clones of yellow nutsedge were analyzed using random amplified polymorphic DNA (RAPD) markers to assess the polymorphism within the species and determine if this approach was suitable for identification of cultivar and wild populations. The RAPD markers unambiguously identified all studied clones. Nei-Li similarities were computed and used in an unweighted pair group method using arithmetic average (UPGMA) cluster analyses. Cultivated and weedy clones were clustered in two groups, but two cultivated clones were more closely related to weedy clones than to cultivated clones. The results showed a high level of genetic variability among the clones tested, particularly among the cultivated ones. Identification of yellow nutsedge cultivars and analysis of genetic diversity within and among weedy populations is possible by using only a small number of primers. In this study, seven selected primers discriminated among the 10 tested clones.
ISSN:0043-1745
1550-2759
DOI:10.1017/S0043174500089487