Synergetic effects of epidermal growth factor and gonadotropins on the cytoplasmic maturation of pig oocytes in a serum–free medium

Cumulus-enclosed or cumulus-free pig oocytes were cultured for 48 h in a serum-free maturation medium, TCM-199B containing 1 mg/ml polyvinylalcohol, supplemented with gonadotropins (PMSG + hCG) and/or epidermal growth factor (EGF), and inseminated with frozen-thawed ejaculated spermatozoa. Fourteen...

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Bibliographic Details
Published in:Zygote (Cambridge) 1995-11, Vol.3 (4), p.345-350
Main Authors: Wanga, Weihua, Niwa, Koji
Format: Article
Language:English
Subjects:
Animals
Cells, Cultured
culture media
Culture Media, Serum-Free
Culture Techniques - methods
cumulus oophorus
Cytoplasm - drug effects
Drug Synergism
EGF
epidermal growth factor
Epidermal Growth Factor - pharmacology
Female
Fertilization in Vitro
Gonadotropin
Gonadotropins - pharmacology
human chorionic gonadotropin
in vitro
Male
maturation
Oocyte cytoplasmic maturation
oocytes
Oocytes - drug effects
Pig
pregnant mare serum gonadotropin
Serum-free medium
Swine
synergism
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Summary:Cumulus-enclosed or cumulus-free pig oocytes were cultured for 48 h in a serum-free maturation medium, TCM-199B containing 1 mg/ml polyvinylalcohol, supplemented with gonadotropins (PMSG + hCG) and/or epidermal growth factor (EGF), and inseminated with frozen-thawed ejaculated spermatozoa. Fourteen hours after insemination, the ability of oocytes to form male pronucleus (MPN) was examined. EGF significantly increased the ability of cumulus-enclosed oocytes to form MPN irrespective of the concentrations (1–100 ng/ml) tested. Gonadotropins had a greater effect than EGF on MPN formation in cumulus-enclosed oocytes, and a synergetic effect was observed when EGF and gonadotropins were added together. However, neither EGF nor gonadotropins exhibited a stimulatory effect on MPN formation in cumulus-free oocytes. These results indicate that the role(s) of both EGF and gonadotropins in stimulating cytoplasmic maturation of pig oocytes is mediated by cumulus.
ISSN:0967-1994
1469-8730
DOI:10.1017/S096719940000277X