3D Confocal Imaging of Pollen

The study of pollen is important in paleoecology, paleontology, archeology, and forensics. The outer shell of a pollen grain bears characteristic features that help in determining the species or genus of origin. To view the pollen grain from several angles in a light microscope, the sample is usuall...

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Bibliographic Details
Published in:Microscopy today 2010-03, Vol.18 (2), p.26-28
Main Authors: Vitha, Stanislav, Bryant, Vaughn M., Zwa, Amen, Holzenburg, Andreas
Format: Magazinearticle
Language:English
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Summary:The study of pollen is important in paleoecology, paleontology, archeology, and forensics. The outer shell of a pollen grain bears characteristic features that help in determining the species or genus of origin. To view the pollen grain from several angles in a light microscope, the sample is usually mounted in glycerol and rolled on the microscopic slide by pushing the coverslip. For teaching, training, and also for establishing databases of pollen images, it is desirable to obtain three-dimensional datasets that allow rotation and viewing from any angle and reslicing in the computer.
ISSN:1551-9295
2150-3583
DOI:10.1017/S1551929510000039