Isotope Dilution High-Performance Liquid Chromatography/Tandem Mass Spectrometry Method for Quantifying Urinary Metabolites of Atrazine, Malathion, and 2,4-Dichlorophenoxyacetic Acid

We have developed an isotope dilution high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for quantifying the urinary metabolites of the pesticides atrazine, malathion, and 2,4-dichlorophenoxyacetic acid (2,4-D). Urine samples are extracted with an organic solvent, an...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 1999-08, Vol.71 (16), p.3526-3530
Main Authors: Beeson, M. D, Driskell, W. J, Barr, D. B
Format: Article
Language:English
Subjects:
2,4-Dichlorophenoxyacetic Acid - urine
Atrazine - urine
Biological and medical sciences
Chromatography, High Pressure Liquid - methods
Herbicides - urine
Humans
Insecticides - urine
Malathion - urine
Mass Spectrometry - methods
Medical sciences
Models, Chemical
Pesticides, fertilizers and other agrochemicals toxicology
Toxicology
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Summary:We have developed an isotope dilution high-performance liquid chromatography/tandem mass spectrometry (HPLC/MS/MS) method for quantifying the urinary metabolites of the pesticides atrazine, malathion, and 2,4-dichlorophenoxyacetic acid (2,4-D). Urine samples are extracted with an organic solvent, and the organic fraction is concentrated. The concentrate is then analyzed using HPLC/MS/MS. The limits of detection for the metabolites are less than 0.5 μg/L (parts per billion) in 10 mL of urine, with a high degree of accuracy and precision.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac990130u