Simultaneous Quantification of Biothiols and Deciphering Diverse GSH Stability in Different Live Cells by 19 F-Tag

GSH, Cys, Hcy, and H S are important biothiols and play important roles in the living systems. Quantitative and simultaneous determination of these biothiols under physiological conditions is still a challenge. Herein, we developed an effective F-reactive tag that readily interacts with these four b...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2022-01, Vol.94 (2), p.901-908
Main Authors: Cui, Chao-Yu, Li, Bin, Cheng, Dan, Li, Xia-Yan, Chen, Jia-Liang, Chen, Ya-Ting, Su, Xun-Cheng
Format: Article
Language:English
Subjects:
Animals
Cysteine
Fluorescent Dyes - chemistry
Glutathione
Homocysteine
Spectrometry, Fluorescence - methods
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Summary:GSH, Cys, Hcy, and H S are important biothiols and play important roles in the living systems. Quantitative and simultaneous determination of these biothiols under physiological conditions is still a challenge. Herein, we developed an effective F-reactive tag that readily interacts with these four biothiols for the generation of stable thioether products that have distinguishable F-chemical shifts. These thioester compounds encode the characteristic fingerprint profiles of each biothiols, allowing one to simultaneously quantify and determine these biothiols by 1D F NMR spectroscopy. The intra-/extracellular GSH in live cells was assessed by the established strategy, and remarkable variations in the GSH stability were determined between the normal mammalian cells and cancer cells. It is notable that GSH hydrolyzes efficiently in the out-membrane of the cancer cells and the lysates. In contrast, GSH remains stable in the tested normal cells.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.1c03673