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Bifunctional Single-Molecular Fluorescent Probe: Visual Detection of Mitochondrial SO 2 and Membrane Potential
Mitochondrial membrane potential (MMP) and sulfur dioxide (SO ) significantly affect the mitochondrial state. In this work, and were constructed through side-chain engineering, in which bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave emission was ca...
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Published in: | Analytical chemistry (Washington) 2023-04, Vol.95 (15), p.6287-6294 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Mitochondrial membrane potential (MMP) and sulfur dioxide (SO
) significantly affect the mitochondrial state. In this work,
and
were constructed through side-chain engineering, in which
bearing the poorer hydrophobicity could localize on mitochondria better. Interestingly, short-wave emission was captured due to the sensitive response of
to SO
(LOD = 13.8 nM). Meanwhile, the probe could bind with DNA, presenting enhanced long-wave emission. Encouragingly,
could migrate from mitochondria to the nucleus when MMP was decreased, accompanied by the increase of fluorescence lifetime (9-fold). Hence,
could be used for dual-channel monitoring of mitochondrial SO
and MMP, which showed a completely different pathway from the commercial MMP detectors JC-1/JC-10. The cellular experiments showed that MMP was gradually decreased due to reactive oxygen species-triggered oxidative stress, and the SO
level was up-regulated simultaneously. Overall, this work proposed a new method to investigate and diagnose the mitochondrial-related diseases. |
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ISSN: | 0003-2700 1520-6882 |
DOI: | 10.1021/acs.analchem.2c05021 |