Chimeric Aptamers-Based and MoS 2 Nanosheet-Enhanced Label-Free Fluorescence Polarization Strategy for Adenosine Triphosphate Detection

• Published in: Analytical chemistry (Washington) 2018-11, Vol.90 (22), p.13708-13713
• Main Authors: Fan, Yao-Yao, Mou, Zhao-Li, Wang, Man, Li, Jun, Zhang, Jing, Dang, Fu-Quan, Zhang, Zhi-Qi
• Format: Article
• Language: English
• Subjects: Adenosine Triphosphate - analysis; Aptamers, Nucleotide - chemistry; Berberine - chemistry; Fluorescence Polarization - methods; Limit of Detection; Powder Diffraction
• ISSN: 0003-2700; 1520-6882
• DOI: 10.1021/acs.analchem.8b04107

Adenosine triphosphate (ATP) as a primary energy source plays a unique role in the regulation of all cellular events. The necessity to detect ATP requires sensitive and accurate quantitative analytical strategies. Herein, we present our study of developing a MoS nanosheet-enhanced aptasensor for fluorescence polarization-based ATP detection. A bifunctional DNA strand was designed to consist of chimeric aptamers that recognize and capture ATP and berberine, a fluorescence enhancer. In the absence of ATP, the DNA strand bound to berberine will be hydrolyzed when Exonuclease I (Exo I) is introduced, releasing berberine as a result. In contrast, when ATP is present, ATP aptamer folds into a G-quadruplex structure; thus, the complex can resist degradation by Exo I to maintain berberine for fluorescent detection purpose. In addition, to magnify the fluorescence polarization (FP) signal, MoS nanosheets were also adopted in the system. This nanosheets-enhanced FP strategy is simple and facile which does not require traditional dye-labeled DNA strands and complex operation steps. The developed fluorescence polarization aptasensor showed high sensitivity for the quantification of ATP with a detection limit of 34.4 nM, performing well both in buffer solution and in biological samples.