Physiological and Metabolite Reconfiguration of Olea europaea to Cope and Recover from a Heat or High UV–B Shock

• Published in: Journal of agricultural and food chemistry 2020-10, Vol.68 (41), p.11339-11349
• Main Authors: Dias, Maria Celeste, Santos, Conceição, Silva, Sónia, Pinto, Diana C. G. A, Silva, Artur M. S
• Format: Article
• Language: English
• Subjects: Agricultural and Environmental Chemistry; Chlorophyll - metabolism; Hot Temperature; Lipid Peroxidation - radiation effects; Olea - metabolism; Olea - radiation effects; Oxidative Stress - radiation effects; Photosynthesis - radiation effects; Photosystem II Protein Complex - metabolism
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.0c04719

To understand how olives reconfigure their metabolism to face stress shock episodes, plants from the economically relevant olive (Olea europaea cv. Cobrançosa) were exposed to high UV–B radiation (UV–B, 12 kJ m–2 d–1) or heat shock (HS, 40 °C) for two consecutive days. The physiological responses and some important lipophilic compounds were evaluated immediately (day 0) and 30 days after UV–B or HS episodes. Both treatments induced a reduction of the olive physiological performance, particularly increasing cell membrane damages and proline pool and at the same time reducing chlorophyll levels, the quantum yield of photosystem II (ΦPSII), and the efficiency of excitation energy capture by open photosystem II (PSII) reaction centers (F′ v/F′ m). Nevertheless, the HS episode caused more adverse effects, additionally reducing the pool of protective pigments (carotenoids) and the maximum efficiency of PSII (with F 0 increase). In the UV–B treatment, despite the higher lipid peroxidation, the activation of some stress protective mechanisms (e.g., increase of NPQ and carotenoids and remobilization of some metabolites, such as phytol and proline) might have contributed to avoiding photoinhibition. Thirty days after stress relief, the performance of olives from both treatments recovered similarly, in part due to the metabolites’ adjustments that contributed to strengthened stress protection (an increase of long-chain alkanes) and provided energy (through the use of soluble sugars, mannitol, and myo-inositol) for re-establishment. Other metabolites, like anthocyanins and squalene, also have an important role in responding specifically to HS or UV–B recovery for helping in the oxidative damage control. These data contribute to understanding how young olive plants may deal with climatic episodes when being transferred from nurseries to field orchards, under the actual context of climate change.