Multiple Mutations in SDHB and SDHC 2 Subunits Confer Resistance to the Succinate Dehydrogenase Inhibitor Cyclobutrifluram in Fusarium fujikuroi

is one of the dominant phytopathogenic fungi causing rice bakanae disease worldwide. Cyclobutrifluram is a novel succinate dehydrogenase inhibitor (SDHI), which shows strong inhibitory activity against . The baseline sensitivity of 112 to cyclobutrifluram was determinated with a mean EC value of 0.0...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2023-03, Vol.71 (8), p.3694-3704
Main Authors: Xue, Zhaolin, Zhong, Shan, Shen, Jinghuan, Sun, Ye, Gao, Xuheng, Wang, Xiangyang, Li, Feng, Lu, Liang, Liu, Xili
Format: Article
Language:English
Subjects:
Fungicides, Industrial - pharmacology
Fusarium - genetics
Molecular Docking Simulation
Mutation
Oryza - microbiology
Plant Diseases - microbiology
Succinate Dehydrogenase - genetics
Succinic Acid
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Summary:is one of the dominant phytopathogenic fungi causing rice bakanae disease worldwide. Cyclobutrifluram is a novel succinate dehydrogenase inhibitor (SDHI), which shows strong inhibitory activity against . The baseline sensitivity of 112 to cyclobutrifluram was determinated with a mean EC value of 0.025 μg/mL. A total of 17 resistant mutants were obtained by fungicide adaptation and displayed equal or slightly weaker fitness than parental isolates, which suggests that the resistance risk of to cyclobutrifluram is medium. A positive cross-resistance was detected between cyclobutrifluram and fluopyram. The amino acid substitutions H248L/Y of FfSdhB and G80R or A83V of FfSdhC conferred cyclobutrifluram resistance in , which was validated by molecular docking and protoplast transformation. The results indicate that the affinity between cyclobutrifluram and FfSdhs obviously decreased after point mutations, causing the resistance of .
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.2c08023