Comprehensive Metabolite Profiling of Two Edible Garcinia Species Based on UPLC-ESI-QTOF-MS E Coupled with Bioactivity Assays

• Published in: Journal of agricultural and food chemistry 2023-05, Vol.71 (19), p.7604-7617
• Main Authors: Lin, Fengke, Kennelly, Edward J, Linington, Roger G, Long, Chunlin
• Format: Article
• Language: English
• Subjects: Chromatography, High Pressure Liquid; Garcinia; Metabolomics; Multivariate Analysis; Plants, Medicinal
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/acs.jafc.2c08372

In China, the endemic species and native are known as edible and medicinal plants. However, a systematic metabolomic and bioactivity evaluation of different plant parts from both species is lacking. In this study, comprehensive investigations of 11 plant parts of and 10 of employing UPLC-ESI-QTOF-MS -based metabolomic analysis in conjunction with three bioactivity assays were undertaken. A customized chemotaxonomic-based in-house library containing 6456 compounds was constructed and coupled to the Progenesis QI informatic platform for metabolite annotations. From these two species, a total of 235 constituents were characterized using multiple criteria. Differences in metabolite profiles between the plant parts within each species were uncovered using multivariate analysis. Based on orthogonal partial least-squares discriminant analysis (OPLS-DA), 23 markers were identified as highly differential metabolites from and 20 from . Comparative assessment of the biological assays revealed the activity variations among different plant parts. The seeds of both species and latex exhibited excellent cytotoxic and antibacterial activities, while roots and arils showed strong anti-inflammatory effects. -plot analysis identified 26 potential biomarkers for the observed activities, including the known cytotoxic agent cycloxanthochymol and the anti-inflammatory compound garcimultiflorone B, which likely explains some of the potent observed bioactivity.