Development and Validation of a Lateral Flow Immunoassay for the Rapid Screening of Okadaic Acid and All Dinophysis Toxins from Shellfish Extracts

A single-step lateral flow immunoassay was developed and validated to detect okadaic acid (OA) and dinophysis toxins (DTXs), which cause diarrhetic shellfish poisoning. The performance characteristics of the test were investigated, in comparison to reference methods (liquid chromatography tandem mas...

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Bibliographic Details
Published in:Journal of agricultural and food chemistry 2015-09, Vol.63 (38), p.8574-8583
Main Authors: Jawaid, Waqass, Meneely, Julie P, Campbell, Katrina, Melville, Karrie, Holmes, Stephen J, Rice, Jennifer, Elliott, Christopher T
Format: Article
Language:English
Subjects:
Animals
Bivalvia - chemistry
Immunoassay - methods
Marine Toxins - analysis
Okadaic Acid - analysis
Ostreidae - chemistry
Pectinidae - chemistry
Shellfish - analysis
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Summary:A single-step lateral flow immunoassay was developed and validated to detect okadaic acid (OA) and dinophysis toxins (DTXs), which cause diarrhetic shellfish poisoning. The performance characteristics of the test were investigated, in comparison to reference methods (liquid chromatography tandem mass spectrometry and/or bioassay), using both spiked and naturally contaminated shellfish. A portable reader was used to generate a qualitative result, indicating the absence or presence of OA-group toxins, at concentrations relevant to the maximum permitted level (MPL). Sample homogenates could be screened in 20 min (including extraction and assay time) for the presence of free toxins (OA, DTX1, DTX2). DTX3 detection could be included with the addition of a hydrolysis procedure. No matrix effects were observed from the species evaluated (mussels, scallops, oysters, and clams). Results from naturally contaminated samples (n = 72) indicated no false compliant results and no false noncompliant results at
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.5b01254