Bovine Serum Albumin-Conjugated Red Emissive Gold Nanocluster as a Fluorescent Nanoprobe for Super-resolution Microscopy

The gold nanocluster (GNC), because of its interesting photoluminescence properties and easy renal clearance from the body, has tremendous biomedical applications. Unfortunately, it has never been explored for super-resolution microscopy (SRM). Here, we present a protein-conjugated red emissive GNC...

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Bibliographic Details
Published in:The journal of physical chemistry letters 2020-07, Vol.11 (14), p.5741-5748
Main Authors: Yadav, Aditya, Verma, Navneet C, Rao, Chethana, Mishra, Pushpendra M, Jaiswal, Amit, Nandi, Chayan K
Format: Article
Language:English
Subjects:
Animals
Cattle
Fluorescent Dyes - chemistry
Gold - chemistry
HeLa Cells
Humans
Lysosomes - metabolism
Metal Nanoparticles - chemistry
Microscopy, Fluorescence - methods
Physical Insights into Materials and Molecular Properties
Serum Albumin, Bovine - chemistry
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Summary:The gold nanocluster (GNC), because of its interesting photoluminescence properties and easy renal clearance from the body, has tremendous biomedical applications. Unfortunately, it has never been explored for super-resolution microscopy (SRM). Here, we present a protein-conjugated red emissive GNC for super-resolution radial fluctuation (SRRF) of the lysosome in HeLa cells. The diameter of the lysosome obtained in SRRF is ∼59 nm, which is very close to the original diameter of the smallest lysosome in HeLa cells. Conjugation of protein to GNC aided in the specific labeling of the lysosome. We hope that GNC not only will replace some of the common dyes used in SRM but due to its electron beam contrast could also be used as a multimodal probe for several other correlative bioimaging techniques.
ISSN:1948-7185
1948-7185
DOI:10.1021/acs.jpclett.0c01354