Navigated Delivery of Peptide to the Nanopore Using In-Plane Heterostructures of MoS 2 and SnS 2 for Protein Sequencing

• Published in: The journal of physical chemistry letters 2022-05, Vol.13 (17), p.3863-3872
• Main Authors: Si, Wei, Yuan, Runyi, Wu, Gensheng, Kan, Yajing, Sha, Jingjie, Chen, Yunfei, Zhang, Yin, Shen, Yang
• Format: Article
• Language: English
• ISSN: 1948-7185; 1948-7185
• DOI: 10.1021/acs.jpclett.2c00533

The impressive success of DNA sequencing using nanopores makes it possible to realize nanopore based protein sequencing. Well-controlled capture and linear movement of the protein are essential for accurate nanopore protein sequencing. Here, by taking advantage of different binding affinities of protein to two isomorphic materials, we theoretically designed a heterostructual platform for delivering the unfolded peptide to the nanopore sensing region. Due to the stronger binding between the peptide and SnS compared to MoS , the peptide would adsorb to the SnS nanostripe and keep its threadlike conformation in the MoS /SnS /MoS heterostructure. Through switching the direction of the applied electric field in real time, the peptide was strategically driven to move along the designed path to the target nanopore. The ionic current blockades were also found to be different as the compositions of the peptide were changed, indicating the possibility for differentiating different peptides using this platform.