Large and Externally Positioned Ligand-Coated Nanopatches Facilitate the Adhesion-Dependent Regenerative Polarization of Host Macrophages

Macrophages can associate with extracellular matrix (ECM) demonstrating nanosequenced cell-adhesive RGD ligand. In this study, we devised barcoded materials composed of RGD-coated gold and RGD-absent iron nanopatches to show various frequencies and position of RGD-coated nanopatches with similar are...

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Published in:Nano letters 2020-10, Vol.20 (10), p.7272-7280
Main Authors: Min, Sunhong, Jeon, Yoo Sang, Choi, Hyojun, Khatua, Chandra, Li, Na, Bae, Gunhyu, Jung, Hee Joon, Kim, Yuri, Hong, Hyunsik, Shin, Jeongeun, Ko, Min Jun, Ko, Han Seok, Kim, Taesoon, Moon, Jun Hwan, Song, Jae-Jun, Dravid, Vinayak P, Kim, Young Keun, Kang, Heemin
Format: Article
Language:English
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Summary:Macrophages can associate with extracellular matrix (ECM) demonstrating nanosequenced cell-adhesive RGD ligand. In this study, we devised barcoded materials composed of RGD-coated gold and RGD-absent iron nanopatches to show various frequencies and position of RGD-coated nanopatches with similar areas of iron and RGD-gold nanopatches that maintain macroscale and nanoscale RGD density invariant. Iron patches were used for substrate coupling. Both large (low frequency) and externally positioned RGD-coated nanopatches stimulated robust attachment in macrophages, compared with small (high frequency) and internally positioned RGD-coated nanopatches, respectively, which mediate their regenerative/anti-inflammatory M2 polarization. The nanobarcodes exhibited stability in vivo. We shed light into designing ligand-engineered nanostructures in an external position to facilitate host cell attachment, thereby eliciting regenerative host responses.
ISSN:1530-6984
1530-6992
DOI:10.1021/acs.nanolett.0c02655