Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker

This search aimed to investigate the genetic diversity of 17 different phenotypes collected from a Lepidium sativum plant from different regions of Iran province and investigate kinship relationships. DNA was extracted from leaf samples using the CTAB method. To evaluate 8 RAPD primers’ genetic dive...

Full description

Saved in:
Bibliographic Details
Published in:ACS agricultural science & technology 2021-12, Vol.1 (6), p.684-690
Main Authors: Mortazavi Moghadam, Faezeh Alsadat, Qaderi, Ardeshir, Sharifi-Sirchi, Gholam-Reza
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3
cites cdi_FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3
container_end_page 690
container_issue 6
container_start_page 684
container_title ACS agricultural science & technology
container_volume 1
creator Mortazavi Moghadam, Faezeh Alsadat
Qaderi, Ardeshir
Sharifi-Sirchi, Gholam-Reza
description This search aimed to investigate the genetic diversity of 17 different phenotypes collected from a Lepidium sativum plant from different regions of Iran province and investigate kinship relationships. DNA was extracted from leaf samples using the CTAB method. To evaluate 8 RAPD primers’ genetic diversity, a total of 70 bands were amplified, 47 polymorphic bands and 23 single bands, with the highest number of OPJ-10 primers with 13 bands and the lowest number of OPA-09 and OPB-01 primers with five bands. The OPJ-10 primer had the highest EMR values of 10.8 and β of 0.9, and this value was zero in the EMR, β primers OPB-01 and OPA-09. In cluster analysis using the UPGMA method (with a genetic distance of 0.84), the six studied populations were classified. The highest genetic distance was obtained between Kerman and Joupar populations (with a genetic similarity of 0.69), which can be used to produce hybrid cultivars and heterosis productivity.
doi_str_mv 10.1021/acsagscitech.1c00182
format article
fullrecord <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_acsagscitech_1c00182</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>c347412552</sourcerecordid><originalsourceid>FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3</originalsourceid><addsrcrecordid>eNp9kM1uwjAQhK2qlYoob9CDj-0h1Ov8OUcElCJRFaH2HDnOmpqGBNkJEg_Q9675OXDqaVY7-41WQ8gjsCEwDi9SObl2yrSovoegGAPBb0iPJxkPIIv57dV8TwbObRhjPIaQxaJHfqd7WXWyNU1NG01nWGNrFJ2YPVpn2sNxCSldNruuOl05-rTAnSlNt6XOb_ZeF8Nnuqxk3dJxU1WoWiypts3Wx2iNFr2xwvUJ9nFzK2taHOhqtJzQd2l_0D6QOy0rh4OL9snX6_Rz_BYsPmbz8WgRSJ7xNkjLAoqoQIikiAQkSahTngBiKSEsBRcqFiVXwAAwEwnnUiexTlKQ3lclhn0SnXOVbZyzqPOdNVtpDzmw_Nhmft1mfmnTY-yMeTffNJ2t_ZP_I38SaXyv</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read &amp; Publish Agreement 2022-2024 (Reading list)</source><creator>Mortazavi Moghadam, Faezeh Alsadat ; Qaderi, Ardeshir ; Sharifi-Sirchi, Gholam-Reza</creator><creatorcontrib>Mortazavi Moghadam, Faezeh Alsadat ; Qaderi, Ardeshir ; Sharifi-Sirchi, Gholam-Reza</creatorcontrib><description>This search aimed to investigate the genetic diversity of 17 different phenotypes collected from a Lepidium sativum plant from different regions of Iran province and investigate kinship relationships. DNA was extracted from leaf samples using the CTAB method. To evaluate 8 RAPD primers’ genetic diversity, a total of 70 bands were amplified, 47 polymorphic bands and 23 single bands, with the highest number of OPJ-10 primers with 13 bands and the lowest number of OPA-09 and OPB-01 primers with five bands. The OPJ-10 primer had the highest EMR values of 10.8 and β of 0.9, and this value was zero in the EMR, β primers OPB-01 and OPA-09. In cluster analysis using the UPGMA method (with a genetic distance of 0.84), the six studied populations were classified. The highest genetic distance was obtained between Kerman and Joupar populations (with a genetic similarity of 0.69), which can be used to produce hybrid cultivars and heterosis productivity.</description><identifier>ISSN: 2692-1952</identifier><identifier>EISSN: 2692-1952</identifier><identifier>DOI: 10.1021/acsagscitech.1c00182</identifier><language>eng</language><publisher>American Chemical Society</publisher><ispartof>ACS agricultural science &amp; technology, 2021-12, Vol.1 (6), p.684-690</ispartof><rights>2021 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3</citedby><cites>FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3</cites><orcidid>0000-0002-1162-8296</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids></links><search><creatorcontrib>Mortazavi Moghadam, Faezeh Alsadat</creatorcontrib><creatorcontrib>Qaderi, Ardeshir</creatorcontrib><creatorcontrib>Sharifi-Sirchi, Gholam-Reza</creatorcontrib><title>Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker</title><title>ACS agricultural science &amp; technology</title><addtitle>ACS Agric. Sci. Technol</addtitle><description>This search aimed to investigate the genetic diversity of 17 different phenotypes collected from a Lepidium sativum plant from different regions of Iran province and investigate kinship relationships. DNA was extracted from leaf samples using the CTAB method. To evaluate 8 RAPD primers’ genetic diversity, a total of 70 bands were amplified, 47 polymorphic bands and 23 single bands, with the highest number of OPJ-10 primers with 13 bands and the lowest number of OPA-09 and OPB-01 primers with five bands. The OPJ-10 primer had the highest EMR values of 10.8 and β of 0.9, and this value was zero in the EMR, β primers OPB-01 and OPA-09. In cluster analysis using the UPGMA method (with a genetic distance of 0.84), the six studied populations were classified. The highest genetic distance was obtained between Kerman and Joupar populations (with a genetic similarity of 0.69), which can be used to produce hybrid cultivars and heterosis productivity.</description><issn>2692-1952</issn><issn>2692-1952</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><recordid>eNp9kM1uwjAQhK2qlYoob9CDj-0h1Ov8OUcElCJRFaH2HDnOmpqGBNkJEg_Q9675OXDqaVY7-41WQ8gjsCEwDi9SObl2yrSovoegGAPBb0iPJxkPIIv57dV8TwbObRhjPIaQxaJHfqd7WXWyNU1NG01nWGNrFJ2YPVpn2sNxCSldNruuOl05-rTAnSlNt6XOb_ZeF8Nnuqxk3dJxU1WoWiypts3Wx2iNFr2xwvUJ9nFzK2taHOhqtJzQd2l_0D6QOy0rh4OL9snX6_Rz_BYsPmbz8WgRSJ7xNkjLAoqoQIikiAQkSahTngBiKSEsBRcqFiVXwAAwEwnnUiexTlKQ3lclhn0SnXOVbZyzqPOdNVtpDzmw_Nhmft1mfmnTY-yMeTffNJ2t_ZP_I38SaXyv</recordid><startdate>20211220</startdate><enddate>20211220</enddate><creator>Mortazavi Moghadam, Faezeh Alsadat</creator><creator>Qaderi, Ardeshir</creator><creator>Sharifi-Sirchi, Gholam-Reza</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-1162-8296</orcidid></search><sort><creationdate>20211220</creationdate><title>Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker</title><author>Mortazavi Moghadam, Faezeh Alsadat ; Qaderi, Ardeshir ; Sharifi-Sirchi, Gholam-Reza</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Mortazavi Moghadam, Faezeh Alsadat</creatorcontrib><creatorcontrib>Qaderi, Ardeshir</creatorcontrib><creatorcontrib>Sharifi-Sirchi, Gholam-Reza</creatorcontrib><collection>CrossRef</collection><jtitle>ACS agricultural science &amp; technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Mortazavi Moghadam, Faezeh Alsadat</au><au>Qaderi, Ardeshir</au><au>Sharifi-Sirchi, Gholam-Reza</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker</atitle><jtitle>ACS agricultural science &amp; technology</jtitle><addtitle>ACS Agric. Sci. Technol</addtitle><date>2021-12-20</date><risdate>2021</risdate><volume>1</volume><issue>6</issue><spage>684</spage><epage>690</epage><pages>684-690</pages><issn>2692-1952</issn><eissn>2692-1952</eissn><abstract>This search aimed to investigate the genetic diversity of 17 different phenotypes collected from a Lepidium sativum plant from different regions of Iran province and investigate kinship relationships. DNA was extracted from leaf samples using the CTAB method. To evaluate 8 RAPD primers’ genetic diversity, a total of 70 bands were amplified, 47 polymorphic bands and 23 single bands, with the highest number of OPJ-10 primers with 13 bands and the lowest number of OPA-09 and OPB-01 primers with five bands. The OPJ-10 primer had the highest EMR values of 10.8 and β of 0.9, and this value was zero in the EMR, β primers OPB-01 and OPA-09. In cluster analysis using the UPGMA method (with a genetic distance of 0.84), the six studied populations were classified. The highest genetic distance was obtained between Kerman and Joupar populations (with a genetic similarity of 0.69), which can be used to produce hybrid cultivars and heterosis productivity.</abstract><pub>American Chemical Society</pub><doi>10.1021/acsagscitech.1c00182</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-1162-8296</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 2692-1952
ispartof ACS agricultural science & technology, 2021-12, Vol.1 (6), p.684-690
issn 2692-1952
2692-1952
language eng
recordid cdi_crossref_primary_10_1021_acsagscitech_1c00182
source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
title Evaluation of Genetic Diversity of 17 Populations (Lepidium sativum L.) Plant Collected from Different Regions of Iran by RAPD Marker
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-25T23%3A10%3A10IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Evaluation%20of%20Genetic%20Diversity%20of%2017%20Populations%20(Lepidium%20sativum%20L.)%20Plant%20Collected%20from%20Different%20Regions%20of%20Iran%20by%20RAPD%20Marker&rft.jtitle=ACS%20agricultural%20science%20&%20technology&rft.au=Mortazavi%20Moghadam,%20Faezeh%20Alsadat&rft.date=2021-12-20&rft.volume=1&rft.issue=6&rft.spage=684&rft.epage=690&rft.pages=684-690&rft.issn=2692-1952&rft.eissn=2692-1952&rft_id=info:doi/10.1021/acsagscitech.1c00182&rft_dat=%3Cacs_cross%3Ec347412552%3C/acs_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a292t-7db1b4be14a8481663f7261eeda13d828c58d2c1011e98622af65f671aa13cde3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true