In situ Synthesis of Fluorescent Gold Nanoclusters by Nontumorigenic Microglial Cells

To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in hea...

Full description

Saved in:
Bibliographic Details
Published in:ACS applied materials & interfaces 2016-08, Vol.8 (33), p.21221-21227
Main Authors: West, Abby L, Schaeublin, Nicole M, Griep, Mark H, Maurer-Gardner, Elizabeth I, Cole, Daniel P, Fakner, Alexis M, Hussain, Saber M, Karna, Shashi P
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:To date, the directed in situ synthesis of fluorescent gold nanoclusters (AuNCs) has only been demonstrated in cancerous cells, with the theorized synthesis mechanism prohibiting AuNC formation in nontumorigenic cell lines. This limitation hinders potential biostabilized AuNC-based technology in healthy cells involving both chemical and mechanical analysis, such as the direct sensing of protein function and the elucidation of local mechanical environments. Thus, new synthesis strategies are required to expand the application space of AuNCs beyond cancer-focused cellular studies. In this contribution, we have developed the methodology and demonstrated the direct in situ synthesis of AuNCs in the nontumorigenic neuronal microglial line, C8B4. The as-synthesized AuNCs form in situ and are stabilized by cellular proteins. The clusters exhibit bright green fluorescence and demonstrate low (
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.6b06624