Fabrication of BSA@AuNC-Based Nanostructures for Cell Fluoresce Imaging and Target Drug Delivery

• Published in: ACS applied materials & interfaces 2018-03, Vol.10 (10), p.8947-8954
• Main Authors: Ding, Caifeng, Xu, Yujuan, Zhao, Yanan, Zhong, Hua, Luo, Xiliang
• Format: Article
• Language: English
• Subjects: Animals; Doxorubicin; Drug Delivery Systems; Gold; Nanostructures; Serum Albumin, Bovine
• ISSN: 1944-8244; 1944-8252
• DOI: 10.1021/acsami.7b18493

Drug delivery which can offer efficient and localized drug transportation together with imaging capabilities is highly demanded in the development of cancer theranostic approaches. Herein, we report the construction of bovine serum albumin (BSA) gold nanoclusters (BSA@AuNCs) for cell fluoresce imaging and target drug delivery. BSA@AuNCs were modified with cyclic arginine-glycine-aspartate with the product RGD–BSA@AuNCs to enhance cell internalization of the nanoclusters. Furthermore, doxorubicin hydrochloride or doxorubicin (DOX), a widely used chemotherapy drug, was also used to modify RGD–BSA@AuNCs. The final design of the DOX/RGD–BSA@AuNC system was constructed through the disulfide bond. The physical microstructure and biological characterization of the BSA@AuNCs were realized through high-resolution transmission electron microscopy and confocal laser fluorescence microscopy. As the disulfide bonds were cleaved by glutathione in cancer cells, DOX-SH molecules were released from the nanosystem to inhibit the growth of cancer cells. The as-prepared DOX/RGD–BSA@AuNC system can be used not only to deliver drug but also to achieve the antitumor effect by in vivo imaging, demonstrating its promising applications in cancer treatment.