Multiplex Protein Imaging with Secondary Ion Mass Spectrometry Using Metal Oxide Nanoparticle-Conjugated Antibodies

In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding of life phenomena and disease mechanisms. Herein, we repor...

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Bibliographic Details
Published in:ACS applied materials & interfaces 2020-04, Vol.12 (15), p.18056-18064
Main Authors: Moon, Dae Won, Park, Young Ho, Lee, Sun Young, Lim, Heejin, Kwak, SuHwa, Kim, Minseok S, Kim, Hyunmin, Kim, Eunjoo, Jung, Yebin, Hoe, Hyang-Sook, Kim, Sungjee, Lim, Dong-Kwon, Kim, Chul-Hoon, In, Su-Il
Format: Article
Language:English
Subjects:
Alzheimer Disease - diagnostic imaging
Animals
Antibodies - chemistry
Antibodies - immunology
Antibodies, Immobilized - chemistry
Antibodies, Immobilized - immunology
CA1 Region, Hippocampal - diagnostic imaging
CA1 Region, Hippocampal - metabolism
Disease Models, Animal
Metal Nanoparticles - chemistry
Mice
Mice, Inbred C57BL
Mice, Transgenic
Oxides
Particle Size
Proteins - immunology
Proteins - metabolism
Spectrometry, Mass, Secondary Ion - methods
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Summary:In spite of recent developments in mass spectrometry imaging techniques, high-resolution multiplex protein bioimaging techniques are required to unveil the complex inter- and intracellular biomolecular interactions for accurate understanding of life phenomena and disease mechanisms. Herein, we report multiplex protein imaging with secondary ion mass spectrometry (SIMS) using metal oxide nanoparticle (MONP)-conjugated antibodies with
ISSN:1944-8244
1944-8252
DOI:10.1021/acsami.9b21800