Recombinant Camel Chymosin Effectively Acts on Milk Coagulation after Immobilization on Eggshell Membranes

To increase the availability of enzymes used in cheese production, the reuse of recombinant camel chymosin immobilized on eggshell membrane using glutaraldehyde was studied and reported for the first time. The catalytic activity, measured as the ability of the chymosin to sediment proteins from skim...

Full description

Saved in:
Bibliographic Details
Published in:ACS food science & technology 2022-05, Vol.2 (5), p.808-814
Main Authors: Kessi, E., Arias, J. L.
Format: Article
Language:English
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53
cites cdi_FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53
container_end_page 814
container_issue 5
container_start_page 808
container_title ACS food science & technology
container_volume 2
creator Kessi, E.
Arias, J. L.
description To increase the availability of enzymes used in cheese production, the reuse of recombinant camel chymosin immobilized on eggshell membrane using glutaraldehyde was studied and reported for the first time. The catalytic activity, measured as the ability of the chymosin to sediment proteins from skim milk, was conserved in the immobilized state compared with the free enzyme. Under the experimental conditions used here, glutaraldehyde did not affect the enzyme activity. The immobilized chymosin remains stable for up to 9 days and can be reused for at least six cycles. In conclusion, chicken eggshell membranes, available as waste material from the poultry industry, are an adequate, useful, and cheap natural matrix for chymosin immobilization.
doi_str_mv 10.1021/acsfoodscitech.1c00407
format article
fullrecord <record><control><sourceid>acs_cross</sourceid><recordid>TN_cdi_crossref_primary_10_1021_acsfoodscitech_1c00407</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>c787199262</sourcerecordid><originalsourceid>FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53</originalsourceid><addsrcrecordid>eNqFkFFLwzAUhYMoOOb-guQPdCZZ0jaPo0wdOATR53KTJltm0kjSCfPXW9ke9Em4cA_n8l0OB6FbSuaUMHoHOtsYu6zdYPRuTjUhnFQXaMJKyQoqOb_8pa_RLOc9IYQJuiBCTtD-xegYlOuhH3ADwXjc7I4hZtfjlbVGD-7T-CNe6iHj2OON8--4ibA9eBjcaIAdTMLrEKJy3n2dzHFW223eGe_xxgSVoDf5Bl1Z8NnMznuK3u5Xr81j8fT8sG6WTwUwWQ-FZcxqUdVUSt4J4NUoO0UFKMrB1kBEWckaNO8qXZaKSi0EZarUbFFzqcRiisrTX51izsnY9iO5AOnYUtL-lNb-La09lzaC7ASO93YfD6kfY_4HfQO5jHcf</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype></control><display><type>article</type><title>Recombinant Camel Chymosin Effectively Acts on Milk Coagulation after Immobilization on Eggshell Membranes</title><source>American Chemical Society:Jisc Collections:American Chemical Society Read &amp; Publish Agreement 2022-2024 (Reading list)</source><creator>Kessi, E. ; Arias, J. L.</creator><creatorcontrib>Kessi, E. ; Arias, J. L.</creatorcontrib><description>To increase the availability of enzymes used in cheese production, the reuse of recombinant camel chymosin immobilized on eggshell membrane using glutaraldehyde was studied and reported for the first time. The catalytic activity, measured as the ability of the chymosin to sediment proteins from skim milk, was conserved in the immobilized state compared with the free enzyme. Under the experimental conditions used here, glutaraldehyde did not affect the enzyme activity. The immobilized chymosin remains stable for up to 9 days and can be reused for at least six cycles. In conclusion, chicken eggshell membranes, available as waste material from the poultry industry, are an adequate, useful, and cheap natural matrix for chymosin immobilization.</description><identifier>ISSN: 2692-1944</identifier><identifier>EISSN: 2692-1944</identifier><identifier>DOI: 10.1021/acsfoodscitech.1c00407</identifier><language>eng</language><publisher>American Chemical Society</publisher><ispartof>ACS food science &amp; technology, 2022-05, Vol.2 (5), p.808-814</ispartof><rights>2022 American Chemical Society</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53</citedby><cites>FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53</cites><orcidid>0000-0002-3165-9831</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27922,27923</link.rule.ids></links><search><creatorcontrib>Kessi, E.</creatorcontrib><creatorcontrib>Arias, J. L.</creatorcontrib><title>Recombinant Camel Chymosin Effectively Acts on Milk Coagulation after Immobilization on Eggshell Membranes</title><title>ACS food science &amp; technology</title><addtitle>ACS Food Sci. Technol</addtitle><description>To increase the availability of enzymes used in cheese production, the reuse of recombinant camel chymosin immobilized on eggshell membrane using glutaraldehyde was studied and reported for the first time. The catalytic activity, measured as the ability of the chymosin to sediment proteins from skim milk, was conserved in the immobilized state compared with the free enzyme. Under the experimental conditions used here, glutaraldehyde did not affect the enzyme activity. The immobilized chymosin remains stable for up to 9 days and can be reused for at least six cycles. In conclusion, chicken eggshell membranes, available as waste material from the poultry industry, are an adequate, useful, and cheap natural matrix for chymosin immobilization.</description><issn>2692-1944</issn><issn>2692-1944</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2022</creationdate><recordtype>article</recordtype><recordid>eNqFkFFLwzAUhYMoOOb-guQPdCZZ0jaPo0wdOATR53KTJltm0kjSCfPXW9ke9Em4cA_n8l0OB6FbSuaUMHoHOtsYu6zdYPRuTjUhnFQXaMJKyQoqOb_8pa_RLOc9IYQJuiBCTtD-xegYlOuhH3ADwXjc7I4hZtfjlbVGD-7T-CNe6iHj2OON8--4ibA9eBjcaIAdTMLrEKJy3n2dzHFW223eGe_xxgSVoDf5Bl1Z8NnMznuK3u5Xr81j8fT8sG6WTwUwWQ-FZcxqUdVUSt4J4NUoO0UFKMrB1kBEWckaNO8qXZaKSi0EZarUbFFzqcRiisrTX51izsnY9iO5AOnYUtL-lNb-La09lzaC7ASO93YfD6kfY_4HfQO5jHcf</recordid><startdate>20220520</startdate><enddate>20220520</enddate><creator>Kessi, E.</creator><creator>Arias, J. L.</creator><general>American Chemical Society</general><scope>AAYXX</scope><scope>CITATION</scope><orcidid>https://orcid.org/0000-0002-3165-9831</orcidid></search><sort><creationdate>20220520</creationdate><title>Recombinant Camel Chymosin Effectively Acts on Milk Coagulation after Immobilization on Eggshell Membranes</title><author>Kessi, E. ; Arias, J. L.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2022</creationdate><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kessi, E.</creatorcontrib><creatorcontrib>Arias, J. L.</creatorcontrib><collection>CrossRef</collection><jtitle>ACS food science &amp; technology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kessi, E.</au><au>Arias, J. L.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Recombinant Camel Chymosin Effectively Acts on Milk Coagulation after Immobilization on Eggshell Membranes</atitle><jtitle>ACS food science &amp; technology</jtitle><addtitle>ACS Food Sci. Technol</addtitle><date>2022-05-20</date><risdate>2022</risdate><volume>2</volume><issue>5</issue><spage>808</spage><epage>814</epage><pages>808-814</pages><issn>2692-1944</issn><eissn>2692-1944</eissn><abstract>To increase the availability of enzymes used in cheese production, the reuse of recombinant camel chymosin immobilized on eggshell membrane using glutaraldehyde was studied and reported for the first time. The catalytic activity, measured as the ability of the chymosin to sediment proteins from skim milk, was conserved in the immobilized state compared with the free enzyme. Under the experimental conditions used here, glutaraldehyde did not affect the enzyme activity. The immobilized chymosin remains stable for up to 9 days and can be reused for at least six cycles. In conclusion, chicken eggshell membranes, available as waste material from the poultry industry, are an adequate, useful, and cheap natural matrix for chymosin immobilization.</abstract><pub>American Chemical Society</pub><doi>10.1021/acsfoodscitech.1c00407</doi><tpages>7</tpages><orcidid>https://orcid.org/0000-0002-3165-9831</orcidid></addata></record>
fulltext fulltext
identifier ISSN: 2692-1944
ispartof ACS food science & technology, 2022-05, Vol.2 (5), p.808-814
issn 2692-1944
2692-1944
language eng
recordid cdi_crossref_primary_10_1021_acsfoodscitech_1c00407
source American Chemical Society:Jisc Collections:American Chemical Society Read & Publish Agreement 2022-2024 (Reading list)
title Recombinant Camel Chymosin Effectively Acts on Milk Coagulation after Immobilization on Eggshell Membranes
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-14T10%3A32%3A16IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-acs_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Recombinant%20Camel%20Chymosin%20Effectively%20Acts%20on%20Milk%20Coagulation%20after%20Immobilization%20on%20Eggshell%20Membranes&rft.jtitle=ACS%20food%20science%20&%20technology&rft.au=Kessi,%20E.&rft.date=2022-05-20&rft.volume=2&rft.issue=5&rft.spage=808&rft.epage=814&rft.pages=808-814&rft.issn=2692-1944&rft.eissn=2692-1944&rft_id=info:doi/10.1021/acsfoodscitech.1c00407&rft_dat=%3Cacs_cross%3Ec787199262%3C/acs_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-a298t-f22fc5781994d5a47781db15ab14af8a056798ac4d7c66b19c5512b6c23849b53%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_id=info:pmid/&rfr_iscdi=true