Manipulating Cellular Activities Using an Ultrasound–Chemical Hybrid Tool

We developed an ultrasound–chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location wi...

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Bibliographic Details
Published in:ACS synthetic biology 2017-11, Vol.6 (11), p.2021-2027
Main Authors: Fan, Ching-Hsiang, Huang, Yao-Shen, Huang, Wei-En, Lee, Albert Alexander, Ho, Sheng-Yang, Kao, Yu-Lin, Wang, Cuei-Ling, Lian, Yen-Ling, Ueno, Tasuku, Andrew Wang, Tsung-Shing, Yeh, Chih-Kuang, Lin, Yu-Chun
Format: Article
Language:English
Subjects:
Animals
Cercopithecus aethiops
COS Cells
HeLa Cells
Humans
Microbubbles
Protein Multimerization
Ultrasonic Waves
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Summary:We developed an ultrasound–chemical hybrid tool to precisely manipulate cellular activities. A focused ultrasound coupled with gas-filled microbubbles was used to rapidly trigger the influx of membrane-impermeable chemical dimerizers into living cells to regulate protein dimerization and location without inducing noticeable toxicity. With this system, we demonstrated the successful modulation of phospholipid metabolism triggered by a short pulse of ultrasound exposure. Our technique offers a powerful and versatile tool for using ultrasound to spatiotemporally manipulate the cellular physiology in living cells.
ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.7b00162