A Rhamnose-Inducible System for Precise and Temporal Control of Gene Expression in Cyanobacteria

Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cya...

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Bibliographic Details
Published in:ACS synthetic biology 2018-04, Vol.7 (4), p.1056-1066
Main Authors: Kelly, Ciarán L, Taylor, George M, Hitchcock, Andrew, Torres-Méndez, Antonio, Heap, John T
Format: Article
Language:English
Subjects:
Binding Sites
DNA-Directed RNA Polymerases - genetics
DNA-Directed RNA Polymerases - metabolism
Escherichia coli - genetics
Escherichia coli Proteins - genetics
Escherichia coli Proteins - metabolism
Gene Expression Regulation, Bacterial
Genes, Reporter
Light
Microorganisms, Genetically-Modified
Plasmids
Promoter Regions, Genetic
Rhamnose - metabolism
Rhamnose - pharmacology
Sigma Factor - genetics
Sigma Factor - metabolism
Synechocystis - drug effects
Synechocystis - genetics
Synechocystis - metabolism
Trans-Activators - genetics
Trans-Activators - metabolism
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Summary:Cyanobacteria are important for fundamental studies of photosynthesis and have great biotechnological potential. In order to better study and fully exploit these organisms, the limited repertoire of genetic tools and parts must be expanded. A small number of inducible promoters have been used in cyanobacteria, allowing dynamic external control of gene expression through the addition of specific inducer molecules. However, the inducible promoters used to date suffer from various drawbacks including toxicity of inducers, leaky expression in the absence of inducer and inducer photolability, the latter being particularly relevant to cyanobacteria, which, as photoautotrophs, are grown under light. Here we introduce the rhamnose-inducible rhaBAD promoter of Escherichia coli into the model freshwater cyanobacterium Synechocystis sp. PCC 6803 and demonstrate it has superior properties to previously reported cyanobacterial inducible promoter systems, such as a non-toxic, photostable, non-metabolizable inducer, a linear response to inducer concentration and crucially no basal transcription in the absence of inducer.
ISSN:2161-5063
2161-5063
DOI:10.1021/acssynbio.7b00435