CRISPR/Cas9-Based Genome Editing in the Filamentous Fungus Glarea lozoyensis and Its Application in Manipulating gloF

• Published in: ACS synthetic biology 2020-08, Vol.9 (8), p.1968-1977
• Main Authors: Wei, Teng-Yun, Wu, Yuan-Jie, Xie, Qiu-Ping, Tang, Jia-Wei, Yu, Zhi-Tuo, Yang, Song-Bai, Chen, Shao-Xin
• Format: Article
• Language: English
• Subjects: Ascomycota - genetics; CRISPR-Cas Systems - genetics; Echinocandins - biosynthesis; Echinocandins - chemistry; Fungal Proteins - genetics; Fungal Proteins - metabolism; Gene Editing - methods; Mutagenesis, Site-Directed; Prolyl Hydroxylases - genetics; Prolyl Hydroxylases - metabolism; RNA, Guide, CRISPR-Cas Systems - metabolism
• ISSN: 2161-5063; 2161-5063
• DOI: 10.1021/acssynbio.9b00491

Glarea lozoyensis is an important industrial fungus that produces the pneumocandin B0, which is used for the synthesis of antifungal drug caspofungin. However, because of the limitations and complications of traditional genetic tools, G. lozoyensis strain engineering has been hindered. In this study, we established an efficient CRISPR/Cas9-based gene editing tool in G. lozoyensis SIPI1208. With this method, gene mutagenesis efficiency in the target locus can be up to 80%, which enables the rapid gene knockout. According to the reports, GloF and Ap-HtyE, proline hydroxylases involved in pneumocandin and Echinocandin B biosynthesis, respectively, can catalyze the proline to generate different ratios of trans-3-hydroxy-l-proline to trans-4-hydroxy-l-proline. Heterologous expression of Ap-HtyE in G. lozoyensis decreased the ratio of pneumocandin C0 to (pneumocandin B0 + pneumocandin C0) from 33.5% to 11% without the addition of proline to the fermentation medium. Furthermore, the gloF was replaced by ap-htyE to study the production of pneumocandin C0. However, the gene replacement has been hampered by traditional gene tools since gloF and gloG, two contiguous genes indispensable in the biosynthesis of pneumocandins, are cotranscribed into one mRNA. With the CRISPR/Cas9 strategy, ap-htyE was knocked in and successfully replaced gloF, and results showed that the knock-in strain retained the ability to produce pneumocandin B0, but the production of pneumocandin C0 was abolished. Thus, this strain displayed a competitive advantage in the industrial production of pneumocandin B0. In summary, this study showed that the CRISPR/Cas9-based gene editing tool is efficient for manipulating genes in G. lozoyensis.