Effects of ultraviolet light on the in vitro assembly of microtubules

Exposure of microtubular protein to ultraviolet light inhibits its assembly into morphologically normal microtubules. This effect appeared to result primarily from damage to the tubulin dimers. The damage consisted of a conformational change, a loss of two free sulfhydryl groups, a production of hig...

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Bibliographic Details
Published in:Biochemistry (Easton) 1984-03, Vol.23 (6), p.1073-1080
Main Authors: Zaremba, Terrye G, LeBon, Thomas R, Millar, David B, Smejkal, Ruthann M, Hawley, Robert J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Brain - ultrastructure
Cattle
Circular Dichroism
Fundamental and applied biological sciences. Psychology
Interphase
Microscopy, Electron
Microtubules - radiation effects
Molecular biophysics
Nephelometry and Turbidimetry
Polymers - metabolism
Structure in molecular biology
Sulfuric Acid Esters - metabolism
Supramolecular structure
Tubulin - radiation effects
Ultracentrifugation
Ultraviolet Rays
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Summary:Exposure of microtubular protein to ultraviolet light inhibits its assembly into morphologically normal microtubules. This effect appeared to result primarily from damage to the tubulin dimers. The damage consisted of a conformational change, a loss of two free sulfhydryl groups, a production of higher molecular weight cross-linked species, and the formation of aggregated amorphous material upon polymerization.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00301a006